Acquisition of a Luxendo MuVi Light Sheet Microscope for the University of Rochester Light Microscopy Shared Resource Laboratories

摘要 本提案旨在获得一台Luxendo MuVi光片显微镜（LSM）， 环境控制单元将安置在光学显微镜共享资源实验室（SRL）中 罗切斯特大学医学中心（URMC）作为共享设备， 多光子和分析成像中心（MAGIC）和先进光中心（Center for Advanced Light） 显微镜和纳米镜（CALMN）。这种显微镜将极大地扩展和增强 这两个成像中心的成像能力，以满足快速三， 精细活标本以及来自不同组织的大型透明组织的三维成像 用户群。如今，研究人员对他们所研究的更完整的图片感兴趣。 具有更高程度的多路复用和吞吐量的模型，而我们目前的成像系统 不足以充分捕捉样品的三维特征， 礼节.引入LSM技术将使许多当前与健康相关的 URMC的研究项目，包括流感，CAR-T疗法，COVID-19， 脊髓损伤、自闭症等，通过显著改善空间和时间成像 能力的使用光片显微镜的技术突破将推动这些 与健康相关的研究，更好地了解复杂的动态和结构， 活的完整标本中的细胞。 高分辨率荧光成像已被证明是一种非常宝贵的核心工具， 生物医学发现的进步。URMC光学显微镜SRL已经看到了快速的 在过去的三年里，随着两位经验丰富的董事的加入， 尖端的成像仪器，大大提高了社区资助的研究， 活动收购光片显微镜将为建立一个国家的新的动力， 在URMC和纽约北部的最先进的综合成像资源， 不断为科学家提供新的途径，以解决与下列方面有关的基本问题： 人类疾病。此外，通过利用本地数据和计算资源， 显微镜SRL将最大限度地发挥光片显微镜技术的影响， 被各种研究计划广泛采用，以进一步促进和推进生物 发现。

ABSTRACT This proposal seeks to obtain a Luxendo MuVi Light Sheet Microscope (LSM) with an environmental control unit to be housed in the Light Microscopy Shared Resource Labs (SRLs) of the University of Rochester Medical Center (URMC) as a shared equipment jointly supported by the Multiphoton and Analytical Imaging Center (MAGIC) and the Center for Advanced Light Microscopy and Nanoscopy (CALMN). This microscope would greatly expand and enhance the imaging capabilities of both imaging centers to fulfill the increasing needs of fast three- dimensional imaging of delicate live specimens as well as large cleared tissues from the diverse user groups. Nowadays, researchers are interested in a more complete picture of their studied models with higher degrees of multiplexing and throughput, while our current imaging systems are insufficient to fully capture the three-dimensional characteristics of samples in fast and gentle manners. Bringing in the LSM technique will significantly benefit many current health-related research programs at URMC, which include studies on Influenza, CAR-T therapy, COVID-19, Spinal Cord Injury, Autism etc., by significantly improving the spatial and temporal imaging capabilities. The technological breakthroughs using light sheet microscopy will advance those health-related research with better understandings of the intricate dynamics and structures of cells within living and intact specimens. High resolution fluorescence imaging has proven an invaluable and central tool for the advancement of biomedical discoveries. The URMC Light Microscopy SRLs have seen a rapid growth in the last three years with the join of two experienced directors introducing three new cutting-edge imaging instruments that have greatly enhanced the community’s funded research activities. Acquisition of a light sheet microscope will add new impetus to establishing a state-of- the-art comprehensive imaging resource at the URMC and the upstate New York that will continuously provide scientists new avenues for bearing on fundamental questions pertaining to human diseases. Additionally, by leveraging the local data and computational resources, the Light Microscopy SRLs will maximize the impact of the light sheet microscopy technique that can be widely adopted by various research programs to further promote and advance biological discoveries.