Core 3: Histology and Tissue Core

摘要 组织学和组织核心的总体目标是作为收集， 保存，注释和分发组织，并为所有人提供高质量的服务和实验支持 项目。鉴于从PC患者获得大量组织的固有困难，我们 设计并建立了一个快速尸检程序，旨在收获原发性肿瘤和器官， 所有死于前列腺癌的个体的转移性肿瘤沉积物。我们制定的程序使我们能够 在死亡后2-3小时内收获并快速冷冻这些组织。这一过程是独特的，具有高度创新性 作为一种组织资源，它使我们能够捕获PC患者的整个病史疾病进展（从 完整的原发性肿瘤和剩余的前体病变直到所有位置的远处转移）。原则上， 我们可以从冰箱中重建整个器官，这使得多个病变的空间分布成为可能， 受影响的地区。迄今为止，联合国监测委员会共进行了113次胰腺癌尸检和2次非癌症尸检。 作为这项工作的一部分，总共进行了115次尸检。所有快速尸检样本都已（和未来 将使用许多程序（北方印迹，南方印迹，RNA-seq，PCR， SDS-PAGE、免疫组织化学、原位杂交、免疫荧光和其他已知的分析。 探针）作为核心功能的一部分。对于本应用程序，该资源将提供病理学 支持和高质量的冷冻样品，冷冻切片的载玻片，福尔马林固定的组织和相关的身体 液体（血液、血清和腹水），用于所有需要组织的项目。组织学核心还将负责 从动物模型中收集、处理和分析肿瘤组织。核心将处理、存档和 切割组织切片并协助染色方案的标准化。核心在分析方面有专长 恶性和良性胰腺病变，包括IPMN、PanIN和PDAC，并将负责 确定基因改变和/或治疗干预对肿瘤组织结构的影响 （治疗反应）和非靶器官（毒理学）。与动物模型和 实验治疗学核心（AMETC），组织学核心将参与存档和编目 患者和动物模型的标本（肿瘤组织、血液和其他器官和体液）。这 核心是一个集中的设备齐全的实验室，用于组织收集，处理，组织学， 免疫组织化学工作。这将有助于所有拟议的项目和目标。本公司的资源和服务 核心将提供给内布拉斯加大学医学中心的人员，以及外部机构的工作 在胰腺癌中。这项服务将带来更多的合作，整合和共享试剂之间的 胰腺癌研究人员参与了该计划项目和UNMC保护伞下的其他项目 胰腺癌研究计划。

Abstract The overall objective of the Histology and Tissue Core is to serve as a central resource for collecting, preserving, annotating and distributing tissues, and provide high quality services and experimental support to all the projects. Given the inherent difficulties in obtaining large quantities of tissues from PC patients, we have devised and instituted a Rapid Autopsy Program that is designed to harvest primary tumor and organs containing all metastatic deposits of tumor from individuals who die of PC. The process we have instituted allows us to harvest and rapidly freeze these tissues within 2-3 hours of death. This process is unique and highly innovative as a tissue resource in that it allows us to capture the entire history disease progression for PC patients (from intact primary tumor and remaining precursor lesions through to distant metastases at all locations). In principle, we can reconstruct the entire organ from the freezer, which enables spatial distribution of multiple lesions and affected areas. To date, UNMC has performed overall 113 pancreas cancer autopsies and 2 non-cancer autopsies for a total of 115 autopsies as part of this effort. All rapid autopsy samples have been (and future samples will be) evaluated for quality using number of procedures (Northern blot, Southern blot, RNA-seq, PCR, SDS-PAGE, immunohistochemistry, in situ hybridization, immunofluorescence and other analyses with known probes) as part of the function of the core. For the purposes of this application, the resource will provide pathology support and high quality frozen samples, slides for frozen sections, formalin fixed tissues, and associated body fluids (blood, serum and ascites) for all projects requiring tissues. The histology core will also be responsible for the collection, processing and analysis of tumor tissues from animal models. The core will process, archive, and cut tissue section and assist in the standardization of staining protocols. The core has expertise in the analysis of malignant and benign pancreatic lesion including IPMNs, PanINs and PDAC and will be responsible to determine the impact of gene alterations and/or therapeutic interventions, on tumor tissue architecture (therapeutic response) and on non-target organs (Toxicology). In collaboration with the Animal Model and Experimental Therapeutics Core (AMETC), the histology core will be involved in the archiving and cataloguing of the specimens (tumor tissue, blood and other organs and body fluids) from patients and animal models. This core is a centralized fully equipped laboratory for the tissue collection, processing, histology, and immunohistochemistry work. It will be useful for all proposed projects and aims. The resource and service of this core will be available for University of Nebraska Medical Center personnel, as well as outside institutions working in pancreatic cancer. This service will bring much collaboration, integration and sharing reagents among the pancreatic cancer researchers involved in this program project and other projects under the umbrella of UNMC Pancreatic Cancer Research Program.