BD FACSymphony S6 cell sorter

项目总结摘要 在过去的15年里，亚利桑那州立大学(ASU)流式细胞术核心设备(FCCF)一直在为 不同研究小组的研究需求。目前，亚利桑那州立大学FCCF定期为这项研究提供服务 6个学术单位的40多个研究实验室的需求。关键的是，这个设施是唯一一个共享的 为亚利桑那州立大学坦佩校区的研究提供流式细胞仪分选能力的核心实验室。 该设施中目前的流式细胞仪BD FACSAria I是在2005年购买的，已经超过了 新教师和现有研究人员所需的细胞分选能力和技术需求 增强的需求。为此，这项提案要求获得资金，以购买BD FACSYMPHONS6单元分选器 配置了5激光、30色系统。这一增强的系统将为用户提供额外的灵敏度和 与目前FCCF中存在的3激光、9色FACSAria I仪器相比，该仪器具有更高的灵活性。此外, 升级后的BD流体系统(这已成为当前FACSAria I系统的限制功能) FACSymphony S6将允许更大的吞吐量和稀有和敏感细胞群体的存活率，这些细胞群体 使用亚利桑那州立大学FCCF的典型研究小组。此外，它的6向和单细胞分选能力 BD FAC Symphony S6与下游单细胞RNA测序平台兼容，这些平台 越来越多地被亚利桑那州立大学FCCF用户使用。最后，屋宇署的无缝整合 带有Baker生物安全柜的FACSymphony S6将允许对病毒转基因细胞进行分选 FCCF目前的FACSARia I系统很难实现这一目标。总的来说， 收购这台新仪器将提供亚利桑那州立大学科学家目前无法获得的资源，并使 并加强NIH资助的研究人员的研究。

PROJECT SUMMARY ABSTRACT Over the last 15 years, the Arizona State University (ASU) Flow Cytometry Core Facility (FCCF) has been serving the research needs of a diverse set of research groups. Currently, the ASU FCCF regularly serves the research needs of over 40 research laboratories across 6 academic units. Critically, this facility serves as the only shared core laboratory that provides flow cytometry sorting capabilities to the researches on the ASU Tempe campus. The current flow cytometer in the facility, the BD FACSAria I, was purchased in 2005 and has outgrown both the cell sorting capacity and technology needs required by new faculty as well as current researchers who have enhanced needs. To that end, this proposal is requesting funds to acquire a BD FACSymphony S6 cell sorter configured with a 5 laser, 30-color system. This enhanced system will provide users additional sensitivity and flexibility when compared to the current 3 laser, 9-color FACSAria I instrument present in the FCCF. In addition, the upgraded fluidics (which has been a limiting feature of the current FACSAria I system) of the BD FACSymphony S6 will allow for greater throughput and survival of rare and sensitive cell populations which are typical of the research groups that use the ASU FCCF. Moreover, the 6-way and single cell sorting capacities of the BD FACSymphony S6 are compatible with the downstream single-cell RNA-sequencing platforms that are becoming increasingly employed by ASU FCCF users. Finally, the seamless integration of the BD FACSymphony S6 with a Baker biological safety cabinet will allow for the sorting of virally transfected cell populations which is not easily achievable with the current FACSAria I system present in the FCCF. Overall, acquisition of this new instrument will provide a resource currently not available to ASU scientists as well enable and enhance the research of NIH-funded investigators.