High Content Imaging System for Microscopy-Based High-Throughput Assays

项目摘要/摘要 高含量显微镜分析允许同时可视化和量化复杂的许多特征 生物系统。在过去的几年里，基于高含量显微镜的筛查越来越多地 被认为是识别和表征新的治疗靶点的一个完整的方法 蜂窝环境。这项技术也被制药公司广泛采用， 通过表型筛选为探针和药物发现提供学术筛选核心。在这里，耶鲁大学中心 分子发现(YCMD)请求资金购买最先进的ImageXpress Micro High 与机器人平台集成的内容筛选系统，以取代过时的系统 制造商已停产。所要求的系统将满足当前项目的需要，并提供 主要的其他获取和分析功能，目前最终无法访问 扩大了许多生物医学研究项目的范围，提高了吞吐量。 YCMD(中心)是一个核心设施，特别适合托管和维护仪器以及 提供高内容的放映服务。该中心专门从事高通量分析开发， 提供各种各样的小分子和基因文库，并维护一个复杂的 基础设施，包括尖端仪器和分析数据库，以支持筛查。 该中心拥有一支经验丰富的团队，在大学的支持下，为不同的 广泛的用户基础，每年支持来自40多个不同实验室的项目 耶鲁大学和非耶鲁大学的院系。值得注意的是，核心支持至少十个主要的高- 年产能显微镜项目，并有成功执行的良好记录 基于图像的转化型生物医学项目。 这项申请得到了9名主修乐器使用者和2名辅修教师的支持 用户，包括七个不同的耶鲁系和一个外部机构。他们的创新 项目涵盖了广泛的基础、翻译和临床研究领域，并解决了许多 治疗适应症包括癌症、心脏病、发育和神经疾病，以及 皮肤病。大多数用户都有NIH的资金，他们的翻译研究也得到了支持 由国防部以及私人和机构基金提供。高含量筛选系统至关重要 为了支持用户的高通量研究，以及ImageXpress Micro系统在此要求 应用程序将是实施和推进当前和未来的一个重大机会 对人类健康有直接影响的变革性生物医学计划。

PROJECT SUMMARY / ABSTRACT High content microscopy assays allow to simultaneously visualize and quantify many features in complex biological systems. Over the past years, high content microscopy-based screening has been increasingly recognized as an integral approach for identifying and characterizing novel therapeutic targets in the cellular context. The technology has also been widely adopted by pharmaceutical companies and academic screening cores for probe and drug discovery via phenotypic screening. Here, Yale Center for Molecular Discovery (YCMD) requests funds to purchase state-of-the-art ImageXpress Micro high content screening system integrated with a robotic platform to replace an outdated system which manufacturer discontinued. The requested system would meet the needs of current projects and offer major additional acquisition and analysis capabilities that are not currently accessible, ultimately expanding the scope and increasing the throughput of many biomedical research programs. YCMD (The Center) is a core facility uniquely suited for hosting and maintaining the instrument and for providing high content screening services. The Center specializes in high-throughput assay development, provides a diverse collection of small molecules and genetic libraries, and maintains a sophisticated infrastructure, including cutting-edge instrumentation and analysis databases, to support screening. Staffed with a highly experienced team and supported by the university, the Center serves a diverse and broad user base, annually supporting projects from over 40 different laboratories from various departments across Yale and non-Yale institutions. Notably, the core supports at least ten major high- throughput microscopy-based projects each year and has a strong track record of successfully executing image-based projects for translational biomedical programs. This application is supported by nine faculty members identified as major instrument users, and two minor users, encompassing seven different Yale departments and an external institution. Their innovative programs span a wide range of basic, translational, and clinical research areas and address many therapeutic indications including cancer, heart diseases, developmental and neurological disorders, and skin diseases. Majority of the users have NIH funding, and their translational research is also supported by the Department of Defense and private and institutional funds. High content screening system is critical to support high-throughput research of the users, and ImageXpress Micro system requested in this application would represent a major opportunity to implement and advance current and future transformative biomedical programs which have direct implications for human health.