Microbiology, Immunology, Animal Modeling and Imaging

微生物学、免疫学、动物建模和成像(迈阿密)核心 约翰霍普金斯大学(JHU)微生物学、免疫学、动物建模和成像研究中心 (迈阿密)核心，主要由约翰霍普金斯结核病中心内的实验室组成 研究中心(CTR)和感染和炎症成像研究中心(Ci3R)将提供广泛的 支持JHU TRAC任务的实验室工具小组。由联席董事Dr. William Bishai和Sanjay Jain，并由另外10名非常有成就的核心教员协助 结核病(TB)和非结核病调查人员，迈阿密核心中心将提供数十年的联合研究 和指导专业知识，以及获得尖端实验室技术以实现培训 (A)微生物学、分子遗传学和生物安全3级 (BSL3)处理结核分枝杆菌；(B)评估宿主对结核病反应的免疫工具 感染和疾病；以及(C)多种临床相关的结核病动物模型(小鼠、豚鼠和 以及使用CT、PET和SPECT进行活体成像的能力。 迈阿密核心中心将协助新调查员(NI)和早期调查员(ESI)制定计划， 执行和解释所有临床前研究的数据，包括结核病的小动物模型、小鼠 育种、先进的全身活体成像方法、代谢组学和单细胞RNA (单链RNA)测序。除了基于实验室的结核分枝杆菌研究的标准方面 微生物学、免疫学和发病机制，迈阿密核心将提供新的附加值 专题(例如，结核病-艾滋病毒混合感染、环境暴露和结核病易感性、结核病的性别差异 敏感性和先进的动物成像)，这些目前不能通过CTR获得，并且 这是JHU TRAC独有的。此外，我们将利用众多独立和机构赠款来 进一步加强JHU TRAC的基础科学能力。 迈阿密核心员工将为所有动物提供科学和技术领导和操作监督- 研究方案的相关研究。迈阿密核心教员还将参加每周一次的TRAC 研讨会系列，提供对结核病发病机制的新研究和新的成像工具的科学关注， 以及TRAC赞助的NI/ESI、TRAC K-Club和K2R Club的写作责任小组， 以及一年一度的结核病科学会议和TRAC数据博览会。一个集中的核心对促进协同至关重要 与JHU的其他TRAC核心配合使用，并提供集成度、效率和易用性，从而最大限度地 对参与JHU TRAC的所有初级和老牌调查人员的价值和效用。总体而言，迈阿密 核心将通过集成专业知识、基础设施和能力来增加显著价值，以实现 提供世界级微生物学、免疫学、动物模型和成像支持的目标是 JHU TRAC，以及为受训人员、初级教员和结核病新手提供研究机会。

ABSTRACT – JHU TRAC Microbiology, Immunology, Animal Modeling and Imaging (MIAMI) Core The Johns Hopkins University (JHU) TRAC Microbiology, Immunology, Animal Modeling and Imaging (MIAMI) Core, comprising laboratories primarily within the Johns Hopkins Center for Tuberculosis Research (CTR) and the Center for Infection and Inflammation Imaging Research (Ci3R), will offer a broad panel of laboratory-based tools to support the mission of the JHU TRAC. Headed by Co-Directors Drs. William Bishai and Sanjay Jain and assisted by a Core faculty of 10 additional highly accomplished tuberculosis (TB) and non-TB investigators, the MIAMI Core will provide decades of combined research and mentoring expertise, as well as access to cutting-edge laboratory technologies to enable training opportunities in and support for TB research in: (a) Microbiology, molecular genetics and biosafety level-3 (BSL3) handling of Mycobacterium tuberculosis; (b) Immunologic tools to assess host responses to TB infection and diseases; and (c) Multiple clinically-relevant animal models of TB (mouse, guinea pig, and rabbit), alongside the capacity to conduct in vivo imaging with CT, PET, and SPECT. The MIAMI Core will assist New Investigators (NI) and Early Stage Investigators (ESI) in planning, executing and interpreting the data from all preclinical studies, including small animal models of TB, mouse breeding, advanced whole-body live in vivo imaging approaches, metabolomics, and single-cell RNA (scRNA) sequencing. In addition to standard aspects of laboratory-based research on M. tuberculosis microbiology, immunology and pathogenesis, the MIAMI core will provide added value by offering novel topics (e.g., TB-HIV co-infection, environmental exposures and TB susceptibility, sex differences in TB susceptibility, and advanced animal imaging), which are not currently available through the CTR and are unique to the JHU TRAC. Furthermore, we will leverage numerous independent and institutional grants to further strengthen basic science capabilities within the JHU TRAC. The MIAMI Core staff will provide scientific and technical leadership and operational oversight to all animal- related studies of the research program. MIAMI Core faculty will also participate in the weekly TRAC Seminar Series, providing a scientific focus on novel research in TB pathogenesis and novel imaging tools, as well as the TRAC-sponsored Writing Accountability Groups for NI/ESI, TRAC K-Club and K2R Club, and annual TB Scientific Meeting and TRAC Data Fair. A centralized Core is essential to promote synergy with the other JHU TRAC Cores and to provide integration, efficiency, and ease of use that will maximize value and utility for all junior and established Investigators involved in the JHU TRAC. Overall, the MIAMI Core will add significant value through integration of expertise, infrastructure, and capabilities to achieve the goal of providing world-class microbiology, immunology, animal models, and imaging support to the JHU TRAC, as well as research opportunities to trainees, junior faculty, and researchers new to TB.