Core C: Epigenetics and Quantitative Proteomics

表观基因组学和定量蛋白质组学核心将为该计划提供高端组学分析 帮助识别和量化组蛋白上的蛋白质表达和翻译后修饰（PTM）的项目 使用质谱，并提供来自各种全基因组实验的信息学分析。专门知识 核心中的基因组学方面包括分析下一代测序和单核苷酸产生的数据 细胞测序技术，绘制各种细胞类型和细胞状态（包括老化细胞）的表观基因组 或衰老细胞，探讨组蛋白PTMs与转录因子结合的关系， 在功能上整合基因组、表观基因组和转录组。在蛋白质组学方面， 具有提供单个和组合组蛋白PTM分析的经验，跟踪细胞信号传导事件 （磷酸蛋白质组学），对中心碳代谢相关分子进行靶向代谢组学， 蛋白质-蛋白质相互作用的鉴定。这将包括进行无标记和稳定同位素标记 在高分辨率Orbitrap质谱仪上进行蛋白质组学和代谢组学实验， 数据分析和生物信息学。所有实验的结果将分发给所有计划项目团队 委员会将鼓励他们进一步互动，并为未来的实验提出有益的建议。核心由 博士Benjamin Garcia和Yemin Lan博士共同带来了多年的免费专业知识，创新和 P01项目的经验。

The Epigenomics and Quantitative Proteomics Core will provide high-end omics analyses for the Program Project to help identify and quantify protein expression and post-translational modifications (PTMs) on histone using mass spectrometry, and to provide informatics analyses from various genome-wide experiments. Expertise in the Core on the genomics side include analyzing data generated from next-generation sequencing and single- cell sequencing technologies, mapping the epigenome of various cell types and cell states including aging cells or cells in senescence, inquiring into the relationship of histone PTMs and transcription factor binding, and functionally integrating the genome, epigenome and transcriptome. On the proteomics side, the Core is well experienced to provide analysis of single and combinatorial histone PTMs, track cellular signaling events (phosphoproteomics), perform targeted metabolomics on central carbon metabolism related molecules and for identification of protein-protein interactions. This will include performing label-free and stable isotope labeled proteomics and metabolomics experiments on high-resolution Orbitrap mass spectrometers, paired with custom data analysis and bioinformatics. Results from all experiments will be disseminated to all Program Project team members to facilitate further interactions and elicit helpful suggestions for future experiments. The Core is led by Dr. Benjamin Garcia and Dr. Yemin Lan who together bring years of complimentary expertise, innovation and experience to the P01 project.