Light Sheet Microscopy for Einstein Shared Facility

该提案寻求资金购买蔡司Light Sheet 7显微镜用于神经细胞 工程和成像核心（NCEI）的玫瑰肯尼迪中心的知识和 阿尔伯特·爱因斯坦医学院的发育障碍研究（IDDR）。 NCEI核心已经作为爱因斯坦研究人员的一个有价值的共享设施， 年在此期间，核心得到了肯尼迪国际减少灾害战略中心的有力财政支助， 由NIH/NICHD中心拨款，神经科学系和学校资助。近几 近年来，光片成像方法的开发和使用显著增加， 生物和生物医学研究，促进全面评价，否则不容易 得到了这为组织图案化、细胞图案化和细胞生物学提供了前所未有的全球视角。 架构、功能集成和远程连接，并可能导致意想不到的 新的见解光片显微镜设施的建立将把这一重要技术带到 爱因斯坦社区第一次，并满足什么是可以理解的显着 需求增长。快速成像大的完整体积的组织和器官的能力将是可行的。 对许多NIH资助的IDDR中心和神经科学研究人员来说， 医学，精神病学和行为科学部门，以及其他。而且都要说明 显微镜将在他们的研究中产生变革，要求检查长距离神经元 途径、体积测定、标记的特定细胞亚群计数和分布，以及 复杂的混合细胞类型阵列。这些研究项目与神经发育相关， 疾病、认知、成瘾、神经可塑性、癫痫、抗癫痫药、动脉粥样硬化和血管 重塑肥胖压力和记忆所提出的显微镜提供的能力 将是爱因斯坦研究人员的一项重要资产，使研究不容易，否则不可能， 提高生产力和增强新的生物医学见解。

This proposal seeks funding to purchase the Zeiss Light Sheet 7 microscope for the Neural Cell Engineering and Imaging Core (NCEI) of the Rose F Kennedy Center for Intellectual and Developmental Disabilities Research (IDDR) at the Albert Einstein College of Medicine (Einstein). The NCEI Core has served as a valuable shared facility for Einstein investigators for close to 35 years. During this the Core has received strong financial support from the Kennedy IDDR Center, funded by NIH/NICHD Center grants, the Department of Neuroscience, and the school. In recent years there has been marked increase in development and use of light sheet imaging approaches to biological and biomedical research, facilitating comprehensive evaluations not otherwise easily obtained. This provides unprecedented global perspective to tissue patterning, cellular architectonics, and functional ensembles and long-range connectivity, and can lead to unanticipated new insights. Establishment of a light sheet microscope facility will bring this important technology to the Einstein community for the first time, and meet what has been understandably a significantly growing demand. The ability to rapidly image large intact volumes of tissues and organs will be invaluable to numerous NIH-funded investigators of the IDDR center and of the Neuroscience, Medicine, Psychiatry and Behavioral Sciences departments, as well as others. In all cases, the microscope will be transformational in their studies that call for examination of long distance neuronal pathways, volume determinations, labeled specific cell sub-population counts and distributions, and complex mixed cell-type arrays. The research projects have relevance to neurodevelopmental disorders, cognition, addiction, neural plasticity, epilepsy, infanticide, atherosclerosis and vascular remodeling, obesity, and stress and memory. The capabilities provided by the proposed microscope would be a vital asset for Einstein researchers, permitting studies not otherwise readily possible, enhancing productivity and potentiating novel biomedical insights.