Core C - Cell Culture and iPS Core (CCiC)

项目概要-核心C 该项目（P01）的细胞培养和iPS核心（CCiC）在宾夕法尼亚大学是独一无二的 以及其他属于P01的机构。CCiC将为P01研究者及其 实验室人员通过相互关联的具体目标，以满足他们的强烈和迫切的需求， 鼠和人食管原代细胞培养物，建立食管细胞系（正常，癌前病变 和癌细胞、正常成纤维细胞和癌症相关成纤维细胞），食管3D培养系统 （器官型3D培养和3D类器官），操纵细胞系中的基因表达（诱导型 逆转录病毒/慢病毒载体、PiggyBac转座子系统、RNA干扰和CRISPR/Cas9 系统）和临床前药物测试，以转化为个性化医疗。CCiC提供丰富的 食管和非食管细胞系（2D和3D）的储存库，其被很好地注释为同一性， 通过和无支原体感染，从而提供质量控制，严谨性和再现性。 此外，CCiC生成标准化协议和定期定向和指令（为所有 项目），这证明是具有成本效益的措施。此外，CCiC将指导和开展服务 与已被遗传重编程为胚胎干细胞的诱导多能干（IPS）细胞相关 细胞样病症和将这些细胞分化为离散的胃肠细胞谱系。通过其服务， 技术，质量控制和时间/成本效益，CCiC推进了这一愿景和使命， P01项目。

PROJECT SUMMARY – CORE C The Cell Culture and iPS Core (CCiC) of this program (P01) project is unique at the University of Pennsylvania and amongst other institutions that are part of the P01. The CCiC will serve the P01 investigators and their laboratory personnel through interrelated Specific Aims to meet their strong and compelling needs related to murine and human esophageal primary cell cultures, establishing esophageal cell lines (normal, premalignant and cancer cells, normal fibroblasts and cancer-associated fibroblasts), esophageal 3D culture systems (organotypic 3D culture and 3D organoids), manipulating gene expression in cell lines (inducible retroviral/lentiviral vectors, the PiggyBac transposon system, RNA interference, and the CRISPR/Cas9 system), and preclinical drug testing for translation to personalized medicine. The CCiC provides a rich repository of esophageal and non-esophageal cell lines (2D and 3D) that are well annotated for identity, passage and free of Mycoplasma infection thereby providing quality control, rigor and reproducibility. Additionally, the CCiC generates standardized protocols and regular orientation and instruction (for all Projects), which prove to be cost-effective measures. Furthermore, the CCiC will instruct and conduct services related to induced pluripotent stem (IPS) cells that have been genetically reprogrammed to an embryonic stem cell-like condition and differentiating such cells to discrete gastrointestinal cell lineages. Through its services, technologies, quality control and time/cost-effectiveness, the CCiC advances the vision and missions of this P01 project.