DEVELOPMENT OF APTAMERS TO RECOGNIZE NOD-LIKE RECEPTORS IN ZEBRAFISH

本项目的目标是开发识别先天免疫细胞中PAMP和DAMP的六种斑马鱼NOD样受体（NLR）的适配体检测试剂。由于其结构多样性和易于合成，适体作为用于特异性结合和检测蛋白质的高亲和力试剂是抗体的有吸引力的替代物。该项目的成功将产生用于检测重要NLR靶标的适体，以检查用于免疫学和传染病研究的斑马鱼模型中的免疫应答。

The goal of this project is to develop aptameric detection reagents for six zebrafish NOD-like receptors (NLRs) that recognize PAMPs & DAMPs in innate immune cells. Aptamers are an attractive alternative to antibodies as a high affinity reagent for specific binding and detection of proteins due to their structural diversity and ease of synthesis. The success of this project will yield aptamers for detecting important NLR targets to examine immune responses in the zebrafish model for immunology and infectious disease studies.