High-speed single molecule detection combining short DNA probes and barcoding bioinformatics for use as a new broad spectrum point-of-care diagnostic

总部位于剑桥的Base 4正在开发一种单分子检测器，每秒可检测多达10亿个单分子，比其他方法快数千倍。由于是固态的，因此大规模生产的道路已经走得很长了，而且消耗品的成本将非常低。不依赖于酶或病原体结合位点，具有快速增加可同时检测的目标病原体数量的显著能力。该项目的目的是生产一种快速，高灵敏度，低成本的多分析物诊断，用于专门针对病原体C的护理点。艰难梭菌、衣原体、淋病和MRSA。该项目将持续3年，并将在24月开始试点试验。该联盟将由Base 4领导，合作伙伴包括桑格威康信托基金会的病原体测序单位、剑桥大学兽医学院和Addenbrookes医院的病理学部门。

Based in Cambridge, Base4 is developing a single-molecule detector with the capacity for detection of up to 1 billion single molecules per second, thousands of times faster than is otherwise possible. Entirely solid-state, there is a well-worn path towards large scale production and the cost of consumables will be very low. Not reliant on enzymes or pathogen binding sites, there is significant capacity to rapidly increase the number of target pathogens which can be tested simultaneously. The aim of this project is to produce a rapid, highly sensitive, low-cost multiple analyte diagnostic for use in point-of-care specifically targeting the pathogens C. difficile, chlamydia, gonorrhea, and MRSA. The project will last 3 years and will result in pilot trials beginning in month 24. The consortium will be led by Base4, with partners including the Pathogen Sequencing Unit at the Sanger Wellcome Trust, the University of Cambridge Veterinary School, and the Pathology Dept at Addenbrookes Hospital.