Method validation for the analysis of cannabinoids in animal specimen and hemp-based animal feed products

项目摘要 该应用程序的目的是开发和验证大麻中大麻素的分析， 衍生食品以及动物标本中。2014年农业法案将种植合法化 和生产工业大麻（大麻含有<0.3%的四氢大麻酚）， 将工业大麻从DEA一级药物名单中移除最近，2018年农业法案 允许增加大麻种植的兴趣，作为一种新的农业商品，在某些情况下， 条文大麻植物及其副产品，被认为具有营养和潜在的 当用作牲畜的饲料来源时具有治疗价值。许多市售大麻 市场上可获得的油和饲料产品很少有关于其大麻素的信息 内容此外，需要在动物标本中开发大麻素筛选， 已知经口给药后其在牛中的分布以及 可食用组织中的损耗。还需要有一个诊断测试， 毒理学实验室，因为宠物经常与大麻衍生产品中毒：在这个 在这种情况下，血浆和尿液将是检测THC暴露的首选标本。成果 将包括：1）扩大FDA调查动物食品或药物相关的能力 2）提高应对大规模动物食品/饲料紧急事件的能力， 3)提高联邦和州动物监测的质量和标准化 食物和饲料。我们已经有了量化大麻素的经验， 补充剂，并已开始在牛血浆中开发该方法。

Project Summary The purpose of this application is develop and validate the analysis of cannabinoids in hemp- derived food products as well as in animal specimen. The 2014 Farm Bill legalized the cultivation and production of industrial hemp (Cannabis sativa containing < 0.3%Tetrahydrocannabinol) and the removal of industrial hemp as DEA Schedule I drug. More recently, the 2018 Farm Bill has allowed for increased interest in hemp cultivation as a novel agricultural commodity under certain provisions. Hemp plants and their by-products, are considered to have nutritional and potentially therapeutic value when used as a feed source in livestock. Many commercially available hemp oils and feed product are available on the market with little information of their cannabinoids content. In addition, there is a need to develop a cannabinoid screen in animal specimen as little is known about their distribution in cattle following oral administration and the time required for depletion in edible tissues. There is also a need to have a diagnostic test available in the toxicology laboratory as pets are regularly intoxicated with cannabis-derived products: in this case, plasma and urine would be specimen of choice to detect exposure to THC. The outcomes will include: 1) the broadening capacity of the FDA to investigate animal food or drug related concerns, 2) an Increased capacity to respond to large-scale animal food/feed emergency events, 3) the improvement in the quality and standardization of Federal and State surveillance of animal food and feed. We already have experience in quantifying cannabinoids in oil and hemp-based supplements and have started to work on developing the method in bovine plasma.