Single molecule DNA/RNA sequencing technology based on a parallel Raman scattering readout in a coupled nanochannel/nanopore system

基于平行拉曼的单分子DNA/RNA传输和拉曼测序技术 纳米通道/纳米孔耦合系统中的散射读数。 摘要 Armonica Technologies，Inc.提议开发一种新型的、高通量、无标签、高度 基于廉价的纳米级图案化和自适应的精确、长读DNA测序平台 集合。平台由纳米通道(横截面尺寸为~100 nm)组成；曲折 由胶体纳米颗粒自组装形成的(缠绕的3D)纳米孔；纳米颗粒屏障 跨纳米沟道放置；以及金属-绝缘体-金属(MIM)场增强结构 纳米沟槽屋顶。在操作中，单链或双链DNA被部分拉伸成 纳米通道中的线性构型被结合到通道中的势垒阻挡并强制 (通过电场)通过屋顶上曲折的纳米孔转移。屋顶上的MIM结构 局部增强外加激光光源的电磁场，允许表面增强相干 反斯托克斯拉曼散射(SECARS)检测单个碱基通过 电磁热点，从而提供单碱基灵敏度和空间定位。独一无二的 单个碱基和表观遗传变体的拉曼光谱允许无标记测序。光学 检测允许大规模并行操作，因为唯一的要求是通过 超过一个光学波长，很容易在制造中完成。的一个重要特点是 平台是多孔的屋顶允许引入寡核苷酸、小蛋白和DNA- 结合/DNA处理酶，允许对DNA进行选择性的操作和修饰 纳米通道。这一第二阶段项目的目标是：开发一种用于凝胶电泳控制的协议 单链DNA易位；设计结构以确保单链DNA通过MIM热点； 对一小段单链DNA进行测序并评估错误率；开发并行光学读取器，AI 分析数据和合适的存储协议。

Single molecule DNA/RNA transport and Raman sequencing technology based on parallel Raman scattering readout in a coupled nanochannel/nanopore system. Abstract Armonica Technologies, Inc. is proposing to develop a novel, high-throughput, label-free, highly accurate, long-read DNA sequencing platform based on inexpensive nanoscale patterning and self- assembly. The platform consists of nanochannels (cross section dimensions of ~ 100nm); tortuous (convoluted 3D) nanopores formed by self-assembly of colloidal nanoparticles; nanoparticle barriers placed across the nanochannels; and a metal-insulator-metal (MIM) field enhancement structure atop the nanochannel roof. In operation, single- stranded- or double-stranded-DNA is partially stretched into a linear configuration in the nanochannels, is blocked at barriers incorporated into the channels and forced (by electric field) to translocate through the tortuous nanopores in the roof. The MIM structure on the roof locally enhances the electromagnetic fields of applied laser sources allowing surface enhanced coherent anti-Stokes Raman scattering (SECARS) detection of individual bases as they pass through the electromagnetic hot spots, thus providing single base sensitivity and spatial localization. The distinct Raman spectra of the individual bases and epigenetic variants allow label-free sequencing. Optical detection allows massively parallel operation since the only requirement is separation of the pores by more than an optical wavelength, easily accomplished in the fabrication. An important feature of the platform is that the porous roofs allow introduction of oligonucleotides, small proteins, and DNA- binding/DNA-processing enzymes, permitting optional manipulation and modification of the DNA in the nanochannels. The goals of this Phase II project are: to develop a protocol for electrophoretic control of the ssDNA translocation; to engineer the structure to ensurethat the ssDNA passes the MIM hot spot; to sequence a short section of ssDNA and evaluate error rates; and to develop parallel optical readout, AI analysis of the data and appropriate storage protocol.