Flow Cytometry Shared Resource

流式细胞术共享资源（FCSR）：摘要 堪萨斯大学医学中心（KUMC）Flow Core是通过NIGMS资助的COBRE建立的 项目（P20 RR 016443/P30 GM 103326）。Flow Core迅速成长为一个重要的组件， 以及堪萨斯大学癌症中心的基础设施 （KUCC）。由于超过三分之一的核心用户是癌症中心的成员，Flow Core被提升为 2018年癌症中心共享资源，由Matthew马约，博士，MBA，FASA，副主任审查后 共享资源。癌症中心领导。流式细胞术共享资源的使命 （FCSR）的目的是为所有KUCC成员提供最先进的流式细胞术和相关技术。 FCSR由科学总监玛丽马凯维奇博士（CB）领导，并拥有两名全职工作人员，一名 技术总监，理查德黑斯廷斯，MS和研究助理，Tykeemi庄园，BS。FSCR房屋 用于细胞分析的BD LSR II和Attune NxT以及用于细胞分选的BD FACSAria IIIu。此外，FCSR Luminex 200用于细胞因子、趋化因子和其他小分子的多重分析。FCSR 协助研究者进行实验设计、方案制定、样品制备、数据采集， 数据分析此外，FCSR在质量保证、培训、 调度、数据存储和数据分析。在过去三年中，该公司提供了以下主要服务： 支持KUCC成员发表25篇论文（5篇论文≥ 10期刊影响因子），41个独立用户， 四项NIH/NIGMS/NCI赠款，一项国防部拨款和四项私人基金会拨款，对分析至关重要 用于NCI资助的临床试验（R37 CA 218118，NCT 01868087）相关研究的人体样本。在 在报告年度（2020年），FCSR支持了34个不同的用户（来自所有三个研究的28个KUCC成员）， 其中75%的项目获得资助，6个项目为非成员）。该基金会的拟议预算（第11年）为： 283,970美元，而CCSG的要求是15014美元。因此，FCSR利用赠款、合同、使用费， 广泛的机构支持，只寻求CCSG基金的5%支持。

FLOW CYTOMETRY SHARED RESOURCE (FCSR): ABSTRACT The University of Kansas Medical Center (KUMC) Flow Core was founded through an NIGMS-funded COBRE program (P20RR016443/P30GM103326) in 2006. The Flow Core quickly grew to be an essential component of the infrastructure of the KUMC research community, as well as The University of Kansas Cancer Center (KUCC). With over one-third of Core users being Cancer Center members, the Flow Core was elevated to a Cancer Center Shared Resource in 2018 after review by Matthew Mayo, PhD, MBA, FASA, Associate Director for Shared Resources. and Cancer Center leadership. The mission of the Flow Cytometry Shared Resource (FCSR) is to provide access to state-of-the-art flow cytometry and related technologies to all KUCC members. The FCSR is led by the Scientific Director, Mary Markiewicz, PhD (CB), and maintains two-full time staff, a Technical Director, Richard Hastings, MS, and a Research Assistant, Tykeemi Manor, BS. The FSCR houses a BD LSR II and Attune NxT for cell analysis and a BD FACSAria IIIu for cell sorting. In addition, the FCSR houses a Luminex 200 for multiplex analysis of cytokines, chemokines, and other small molecules. The FCSR aids investigators in experimental design, protocol development, sample preparation, data acquisition, and data analysis. Additionally, the FCSR has strong procedures in place for quality assurance, training, scheduling, data storage, and data analysis. Over the past three years, the FCSR provided key services in support of 25 publications to KUCC members (five papers ≥ 10 journal impact factor), 41 unique users, and four NIH/NIGMS/NCI grants, one DOD grant, and four private foundation awards, and was essential to analysis of human samples for correlative studies from an NCI-funded clinical trial (R37CA218118, NCT01868087). In the reporting year (2020), the FCSR supported 34 different users (28 KUCC members from all three research programs, of which 75% were funded and six non-members). The proposed budget of the FCSR (Year 11) is $283,970, yet the CCSG request is $15,014. As such, the FCSR leverages grants, contracts, user fees, and extensive institutional support and seeks only 5% support from CCSG funds.