HILIGHT: Highly Integrated Versatile Laser Source enabling two-photon excitation in digital diagnostics and biomedical research

HILIGHT的总体目标是将创新的解决方案应用于多用途光源和系统，作为生物医学和医疗保健应用的工具，以双光子激发荧光寿命测量和成像显微镜为中心。我们提出了两个具体的用例：i)即时数字组织病理学和ii)癌症研究和诊断背景下的生物医学研究。HILIGHT提出了一种小型化和多用途的光脉冲爆发源，以及创新的传感技术，这将使我们能够克服SoA仪器中的限制，这些限制通常限制了双光子显微镜和时间分辨检测的使用，只能在专业实验室进行。我们设想，HILIGHT的创新可以颠覆各个领域和一系列细分市场的活动，包括学术和研究机构、生物技术和医疗设施。这些应用包括旨在提高我们对疾病分子机制的理解，使用体内、体外或3D器官型培养，最终目的是开发更好的工具，用于快速和早期诊断以及更有效的治疗干预。HILIGHT围绕九个工作包构建，涵盖整个价值链，与项目概念一致，立足于强大的技术基础，瞄准生物光子和医学应用领域。HILIGHT联盟由来自欧洲五个不同国家（FR、DE、IT、CH和UK）的五个领先合作伙伴组成，这些合作伙伴来自不同领域，在欧洲合作项目中经验丰富。HILIGHT建立在合作伙伴之间先前和正在进行的几次成功合作的基础上，这为联盟内部的成功互动和工作计划的顺利实施提供了强有力的保证。

The overall objective of HILIGHT is to implement innovative solutions into versatile light sources and systems as tools for biomedical and healthcare applications centred around two-photon excitation fluorescence lifetime measurements and imaging microscopy. We propose two specific use cases: i) instantaneous digital histopathology and ii) biomedical research in the context of cancer research and diagnostics. HILIGHT proposes a miniaturised and versatile optical pulse burst source together with innovative sensing technology that will permit us to overcome those limitations in SoA instrumentation that often confine the use of two-photon microscopy and timeresolved detection to the specialist laboratory. We envisage that the HILIGHT innovations could disrupt activities in various fields and a range of market segments, including academic and research institutions, biotechnology, and medical facilities. These include applications targeted to improve our understanding of the molecular mechanisms of disease using in vivo, ex vivo, or 3D organotypic cultures, with the ultimate aim of developing better tools for fast and early diagnosis and more effective therapeutic interventions. HILIGHT is structured around nine work packages covering the whole value chain, coherently with the concept of the project standing on strong enabling technology foundations and targeting biophotonic and medical application domains. HILIGHT consortium is made up of five leading partners from various fields experienced in European collaborative projects and from five different European countries (FR, DE, IT, CH and UK). HILIGHT is built on several prior and ongoing successful collaborations between partners, which provides a strong guarantee for successful interaction within the consortium, and a smooth implementation of the workplan.