Single-Cell Epigenomics, Transcriptomics, and Bioinformatics Core

摘要 弗吉尼亚大学的PCEN将支持一个联合的生物医学研究核心，以帮助研究人员在两个重要的 领域：1)转录组和表观基因组技术；2)生物信息学。单细胞表观基因组学， 转录学和生物信息学核心将由经验丰富的研究人员(Silvia Medrano，Ph.D. 和内森·谢菲尔德博士。)并配备了训练有素的人员。通过单细胞表观基因组学 和核心的Transcriptomics组件，PCEN将生成新的数据，这将需要大量的单一 细胞和生物信息学资源和专业知识。核心的生物信息学部分将与 并提供计算基础设施和分析支持，以确保 研究人员将这些重要数据转化为知识。通过将所有这些服务集成到一个 核心，我们将确保PCEN调查人员以协调、成本效益和高效的方式开展研究 举止。核心将作为PCEN的两个主要研究项目和试点项目的资源 泰晤士报。此外，该核心将支持UVA和其他更广泛的儿科肾脏研究社区 机构。除了提供各种专门的、创新的和必要的研究服务、技术、 和仪器，核心将为学生、博士后、研究员和年轻科学家提供实践培训 以及咨询服务和使用最先进的研究设备。此外，核心将 为手稿和拨款提案的准备工作提供支持。核心的范围与 本次PCEN的总主题是“肾脏发育和疾病过程中细胞命运的调控”。这个 PCEN的实验方法包括检查表观遗传和转录机制， 控制细胞的命运、可塑性和特性，以及潜在的表型转化的细胞信号 发生在肾脏损伤和修复期间。拟议的核心将提供专门的技术和专业知识 为实现PCEN规定的目标所需要的。

SUMMARY The PCEN at UVA will support a combined biomedical research core to assist investigators in two important areas: 1) Transcriptome and epigenome technologies and 2) Bioinformatics. The Single Cell Epigenomics, Transcriptomics, and Bioinformatics core will be directed by experienced researchers (Silvia Medrano, Ph.D. and Nathan Sheffield, Ph.D.) and staffed with highly trained personnel. Through the Single Cell Epigenomics and Transcriptomics component of the core, the PCEN will generate new data that will require substantial single cell and bioinformatics resources and expertise. The Bioinformatics component of the core will work closely with the single cell component and provide both computational infrastructure and analytical support to ensure that the investigators convert this important data into knowledge. By integrating all these services within a single core, we will ensure that PCEN investigators carry out their research in a coordinated, cost-effective, and efficient manner. The core will serve as a resource for the two PCEN main Research Projects and Pilot Projects at all times. Furthermore, the core will support the broader pediatric kidney research community at UVA and other institutions. In addition to offering a variety of specialized, innovative and essential research services, techniques, and instrumentation, the core will provide hands-on training to students, postdocs, fellows and young scientists as well as consultation services and access to state-of-the-art research equipment. Moreover, the core will provide support for preparation of manuscripts and grant proposals. The scope of the core is well in line with the overall theme of this PCEN that is “Regulation of cell fate during kidney development and disease”. The experimental approaches of the PCEN involve examination of epigenetic and transcriptional mechanisms that control cell fate, plasticity, and identity and the cellular signals underlying the phenotypic transformation that occurs during kidney injury and repair. The proposed core will provide the specialized technologies and expertise needed to accomplish the stated goals of the PCEN.