The Human Lung BioMolecular Multi-Scale Atlas Program (HuBMAP-Lung)

项目总结/摘要 总体研究计划 人类肺生物分子多尺度图谱程序（HuBMAP-Lung） 人类生物分子图谱计划（HuBMAP）正在进入生产阶段，重点是 先进的、高灵敏度和特异性的分析，优先考虑空间生物分子分析。我们的组织图谱 中心（TMC）的建议将集中在一个器官系统，包括两个肺和相关的呼吸道 为了简单起见，我们将其称为“肺”。我们的TMC将生成、标准化和验证 从高含量，高通量成像和`组学技术的广泛数据，以产生系统的人类 高分辨率的肺组织图。我们将从长远来看，一个地图集，作为一个集合的2D和3D地图 包含图像、表格数据、关于不同分辨率的多个位置的事实，以及命名的 以类似于纬度和经度的位置格网的坐标为关键字的对象，以制图方式显示 人类肺部的所有显著特征为了完成这项任务，我们的中心汇集了 来自罗切斯特大学（URMC）、加州大学圣地亚哥分校 （UCSD）、太平洋西北国家实验室（PNNL）、华盛顿大学（UW）和北方大学 卡罗莱纳在查佩尔山（查珀尔山），每一个互补的肺为重点的学术兴趣，知识， 联盟网络连接，并具有国际公认的肺部专业知识和最先进的 生物分子技术我们在第一阶段向HuBMAP数据门户提供的肺部数据主要用于 单核RNA测序，染色质可用性和开始空间转录组学。除了 为了进一步增加这些数据类型中人类多样性的表现，我们TMC的下一阶段将主要 致力于确定细胞和基质的空间组织，不仅由基因表达， 蛋白质，蛋白质修饰，脂质和选择代谢物对解剖学内的特定细胞功能至关重要， 功能niches。集团内外的全球调查工作的价值得到公认， TMC整体组件的具体目标集中在 TMC，所有HuBMAP组成部分之间以及国家和国际研究人员之间的协同作用， 财团。

Project Summary/Abstract Overall Research Plan The Human Lung Biomolecular Multi-Scale Atlas Program (HuBMAP-Lung) The Human Biomolecular Atlas Program (HuBMAP) is entering the production phase, with an emphasis on progressive, high sensitivity and specificity assays, prioritizing spatial biomolecular analysis. Our Tissue Mapping Center (TMC) proposal will focus on one organ system, comprised of two lungs and related respiratory tract (trachea to alveoli) that for simplicity we refer to as the “Lung”. Our TMC will generate, standardize, and validate extensive data from high content, high-throughput imaging and `omics technology to produce systematic human lung tissue maps at high resolution. We will take a long view of an Atlas, as a collection of 2D and 3D maps containing images, tabular data, facts about multiple locations at varying resolution, and indexes of named objects keyed to coordinates of a locational grid analogous to latitude and longitude, to cartographically present the whole range of salient features of the human Lung. To accomplish this task, our center brings together Investigators from five institutions, the University of Rochester (URMC), University of California at San Diego (UCSD), Pacific Northwest National Laboratory (PNNL), University of Washington (UW), and University of North Carolina at Chapel Hill (UNC), each with complementary lung-focused academic interests, knowledge, and inter- consortia network connections and with internationally recognized expertise in lung and state-of-the-art biomolecular technologies. Our Lung data provided to the HuBMAP Data Portal in Phase I has been focused to single nucleus RNA-sequencing, chromatin availability and beginning spatial transcriptomics. In addition to further increasing representation of human diversity in these data types, the next phase of our TMC turns primary effort toward determining spatial organization of cells and matrices defined by not only gene expression but also proteins, protein modifications, lipids and select metabolites critical to specific cell function within anatomical and functional niches. With the recognized value of global investigative efforts within and outside the Consortium, the Specific Aims of the TMC Overall Component concentrate on Communication and Collaboration within the TMC, between all HuBMAP components and synergistically among national and international researchers and Consortia.