MHC Genetics Core

抽象核心C：MHC基因分型核心 MHC基因分型核心的目标是支持遗传特征和育种策略 对猕猴SPF群体的研究，重点是为艾滋病相关研究优化群体。这个 CORE的三个目标建立在CORE现有优势的基础上，并纳入了全基因组的方法 朝着更严格的群体遗传特征发展。 具体目标1：将使用高分辨率对艾滋病研究人员重要的MHC I类等位基因进行基因分型 与威斯康星州NPRC基因组服务公司合作进行测序。175只动物的基因分型 每年都会拿到。 具体目标2：GBS(每年170只动物)、完整外显子组序列(WES)(每年24只动物)和完整 将生成基因组测序(WGS)(每年9只动物)，以提供a)更可靠的目标设置 定义全基因组和特定基因座的印度和中国血统信息，b)血统 用于验证系谱和调查殖民地起源的信息，c)允许 识别携带与艾滋病研究和研究有关的已知和新发现的等位基因的动物 影响传染病发病机制和疫苗效力，以及d)与他人分享遗传数据 更广泛的研究界。 具体目标3：来自目标1和目标2的遗传数据将用于使群体的遗传多样性最大化，而 为SIV/HIV维持足够数量的具有特定MHC I类等位基因和祖先的动物 相关研究。核心C将与SPF监督委员会和核心A合作，将繁殖 使用来自原始殖民地1和2以及新获得的亲本动物的群体 动物。 我们对SNPRC U42支持的SPF印度恒河猴群体的鉴定将提供关键的 基于基因组学的信息，以确保这些有价值的动物对许多艾滋病研究人员来说是可用的 未来的殖民地世代。此外，我们基于基因组学的数据也将对计划的 U42支持的殖民地的扩张。

Abstract– Core C: MHC Genetic Typing Core The objectives of the MHC Genetic Typing Core are to support the genetic characterization and breeding strategy of the rhesus macaque SPF Colony, with a focus towards optimizing the colony for AIDS-related research. The Core's three aims build upon the current strengths of the Core and incorporate genome-wide approaches towards more rigorous genetic characterization of the colony. Specific aim 1: MHC Class I alleles, important to AIDS investigators, will be genotyped using high resolution sequencing in collaboration with Wisconsin NPRC Genomics Services. The genotypes on 175 animals will be obtained each year. Specific aim 2: GbS (170 animals per year), whole exome sequence (WES) (24 animals per year) and whole genome sequencing (WGS) (9 animals per year) will be generated to provide a) a more robust AIMs set defining Indian and Chinese ancestry information both genome-wide and at specific loci, b) parentage information to verify pedigrees and investigate colony origins, c) genotypic information to allow for the identification of animals carrying known and newly identified alleles relevant for AIDS research and impacting infectious disease pathogenesis and vaccine-effectiveness, and d) genetic data to share with the broader research community. Specific aim 3: Genetic data from Aims 1 and 2 will be used to maximize genetic diversity of the colony, while maintaining sufficient numbers of animals with specific MHC Class I alleles and ancestry for SIV/HIV related studies. Core C will work with the SPF Oversight Committee and Core A to assemble breeding groups across our single colony using parent animals from original Colonies 1 and 2 and newly acquired animals. Our characterization of the SNPRC U42-supported SPF Indian rhesus macaque colony will provide critical genomics-based information to ensure the availability of these valuable animals for AIDS researchers for many colony generations to come. Furthermore, our genomics-based data will also be important for the planned expansion of the U42-supported colony.