Viral Testing Core

项目总结/摘要-病毒检测核心 持续、准确地监测所关注的病原体是维持SPF的基本要求 繁殖群体2009年，我们从在非现场实验室进行检测过渡到进行所有筛查检测 在杜兰国家灵长类动物研究中心（TNPRC）的病原体检测和定量中心， （PDQC）。PDQC在行政上位于微生物学部，并在TNPRC成立 2004年PDQC的主要使命是为兽医部门提供诊断服务 用于建立和维持SPF非人灵长类动物群体。第二个使命是提供研究 支持调查员。现场病毒检测能力的发展降低了成本， 消除了样品运输，缩短了周转时间，并提供了一个易于扩展的平台， 检测其他试剂。TNPRC SPF监测计划的质量保证得到以下方面的支持： PDQC参与病原体检测工作组（PDWG）的熟练度小组测试， NIH非人灵长类动物研究联盟（NHPRC）和外部质量保证计划 监督实验室（EQAPOL）Luminex计划。应用程序的这一部分重点介绍 病毒检测核心，用于鉴定和检测靶病毒的技术，检测试剂盒性能和 验证以及当前和未来的测试能力扩展。 该核心的目的是通过对现有育种进行持续的病毒检测，确保殖民地的SPF状态 群体动物和所有后代。对于所有四个目标，SPF 4菌落每年至少检测两次 病毒：D型猿猴逆转录病毒（SRV）、猿猴嗜T淋巴细胞病毒（STLV）、猿猴免疫缺陷病毒 (SIV)和用于SPF 4的疱疹B病毒（BV）。支持的靶向药物以外的殖民地表征 U42赠款将继续通过开发和使用其他病原体的额外检测方法， 潜在的兴趣。

Project Summary/Abstract - Viral Testing Core Continuous, accurate surveillance for agents of concern is an essential requirement for maintenance of a SPF breeding colony. In 2009 we transitioned from testing at off-site laboratories to performing all screening assays at the Tulane National Primate Research Center’s (TNPRC) Pathogen Detection and Quantification Core (PDQC). The PDQC is administratively located in the Division of Microbiology and was established at the TNPRC in 2004. The primary mission of the PDQC is to provide diagnostic services to the Division of Veterinary Medicine for establishing and maintaining SPF nonhuman primate colonies. A secondary mission is to provide research support to investigators. The development of onsite viral testing capabilities has resulted in reduced costs, eliminated shipping of samples, reduced turnaround time, and provided a platform for easy expansion for detection of additional agents. Quality assurance of the TNPRC SPF surveillance program is supported by the participation of the PDQC in proficiency panels testing from the Pathogen Detection Working Group (PDWG) of the NIH Nonhuman Primate Research Consortium (NHPRC) and the External Quality Assurance Program Oversight Laboratory (EQAPOL) Luminex program. This section of the application focuses on the function of the Viral Testing Core, techniques used for identification and detection of the target viruses, assay performance and validation, and current and future expansion of testing capabilities. The Aim of this core is to assure the SPF status of the colony through continued viral testing of existing breeding colony animals and all offspring. The SPF4 colony will be tested a minimum of twice each year for all four target viruses: Type D simian retrovirus (SRV), simian T-lymphotropic virus (STLV), simian immunodeficiency virus (SIV), and herpes B virus (BV) for SPF4. Characterization of the colony beyond the targeted agents supported by the U42 grant will continue through the development and use of additional assays for other pathogens of potential interest.