Core B: Organoid Biobank

核心B -类器官生物样本库 项目摘要 类器官是从茎生长的微观自组织三维细胞结构， 体外祖细胞。这些培养模型概括了它们在体内的许多结构和功能方面， 对口机关。这种多功能的技术已经导致了许多新的人类癌症模型的发展， 其已被广泛验证为具有上级常规2D癌细胞系的能力， 概括了人类癌症生物学。在过去的几年里，CSHL的几名调查人员，包括 该计划的成员，已经走到一起，建立一个独特的类器官生物库。通过一个富有成效的 我们与几个健康中心的临床肿瘤学家、外科医生和病理学家合作， 数以百计的新鲜肿瘤标本，已被改编为原代培养作为类器官。严格 标准操作程序注释了这些培养物的基因组和转录组， 将每个肿瘤类器官分类为分子亚型。重要的是，这个生物库包括许多 胰腺癌和乳腺癌的基底亚型模型，这是该计划的主要重点。期间 在下一个资助周期，核心B将支持所有三个项目使用癌症的类器官模型，这将 包括a）建立基底亚型乳腺癌和胰腺癌新类器官模型B）进行 这些模型的基因组学和转录组学表征c）协助研究者繁殖类器官 d）协助研究者进行定制的测定， 表征类器官形态、迁移和谱系身份。这些宝贵的资源和 这些服务将使人们能够深入研究癌症中的转录和剪接依赖性， 鉴定生物标志物以预测治疗功效。

CORE B – ORGANOID BIOBANK PROJECT SUMMARY Organoids are microscopic self-organizing, three-dimensional cellular structures that are grown from stem and progenitor cells in vitro. These culture models recapitulate many structural and functional aspects of their in vivo counterpart organs. This versatile technology has led to the development of many novel human cancer models, which have been widely validated as having superior capabilities to conventional 2D cancer cell lines in recapitulating human carcinoma biology. Over the last several years, several investigators at CSHL, including members of the Program, have come together to establish a unique Organoid Biobank. Through a productive collaboration with clinical oncologists, surgeons, and pathologists at several health centers, we have obtained hundreds of fresh tumor specimens, which have been adapted as primary cultures as organoids. Rigorous standard operating procedures annotated the genome and transcriptome of these cultures, resulting in the classification of each tumor organoid into molecular subtypes. Importantly, this biobank includes numerous basal-subtype models of pancreatic and breast carcinoma, which are a major focus of this Program. During the next funding cycle, Core B will support all three Projects in the use of organoid models of cancer, which will include a) establishing novel organoid models of basal-subtype breast and pancreatic cancer b) performing genomic and transcriptomic characterization of these models c) assisting investigators in propagating organoid cultures for various perturbation experiments d) assisting investigators in performing customized assays for characterizing organoid morphology, migration, and lineage identity. Collectively, these valuable resources and services will enable a deep investigation of transcriptional and splicing dependencies in cancer and allow for the identification of biomarkers to predict therapeutic efficacy.