Automated Gene Assembly from Codons to Complete Genes and Pathways

从较小的DNA片段组装基因或生成基因突变的组合库的能力是合成生物学领域的基础。它们的制备方法不同，但与其他方法相比，酶组装可产生始终如一的高质量产品，这对于下游工艺改善特性至关重要，如改良菌株或改善蛋白质功能/产量。该项目旨在以高通量、自动化的方式实现组合基因组装，为合成生物学领域的商业化提供一个强大而有价值的工具，并为药物发现和工业应用提供潜在的好处。

The ability to assemble genes from smaller DNA fragments or generate combinatorial libraries of genetic mutations is fundamental to the field of synthetic biology. Different methodologies exist for their fabrication but enzymatic assembly produces a consistently high quality product, compared to other methods, which is essential for downstream processes for improved characteristics such as improved strains or improved protein function/yield. The project aims to achieve combinatorial gene assembly in a high throughput, automatable fashion, delivering a powerful and valuable tool for commercialisation in the synthetic biology arena and providing potential benefits for drug discovery and industrial applications.