ETHANOL EFFECTS ON CNS TISSUE IN VIVO AND IN VITRO

我们将使用电生理学和体内电化学技术， 将急性神经元对乙醇的反应表征为直接膜反应 影响，与突触输入的调节相互作用，或 神经元通路的活动。 酒精暴露对 哺乳动物中枢神经系统的功能将采用 细胞外记录单个神经元的动作电位活动， 以及诱发电位和细胞内记录的膜 特性. 我们将研究乙醇在啮齿动物脑中的作用， 记录来自原位完整脑和oculo中的多个脑移植物， 并采用脑片和离体眼内脑移植物。 我们还将研究乙醇对人类神经元的作用， 异种移植 最后，我们将重点关注遗传变异， 不同的酒精相关行为作为模型系统用于检查急性 乙醇作用、乙醇依赖和乙醇戒断。 新方向 我预计这些科学互动可能会在我的 实验室包括乙醇相关的神经元效应的研究 胎儿乙醇综合征，乙醇对衰老过程的影响， 乙醇参与偏好的细胞机制和遗传学， 依赖，研究生长因子在长期影响中的作用， 乙醇和细胞内机制的膜片钳研究，如 第二信使系统，介导乙醇的影响。

We will use electrophysiological and in vivo electrochemical techniques to characterize acute neuronal responses to ethanol as direct membrane effects, modulatory interactions with synaptic inputs, or alterations in the activity of neuronal pathways. The effects of ethanol exposure on mammalian central nervous system function will be pursued using both extracellular recording of action potential activity from single neurons as well as evoked potentials, and intracellular recording of membrane properties. We will study ethanol effects in rodent brain using in vivo recording from intact brain in situ and in multiple brain grafts in oculo, and using brain slices and extricated intraocular brain grafts in vitro. We will also study ethanol actions on human neurons using in oculo brain xenografts. Finally, we will focus on genetic variants manifesting differential alcohol-related behavior as model systems for examining acute ethanol actions, ethanol dependence and ethanol withdrawal. New directions which I anticipate that these scientific interactions may take in my laboratory include studies of the neuronal effects of ethanol associated with fetal ethanol syndrome, the effect of ethanol on aging processes, the cellular mechanisms and genetics of ethanol involved in preference and dependence, studies of the role of growth factors in long-term effects of ethanol, and patch-clamp studies of the intracellular mechanisms, such as second messenger systems, mediating the effects of ethanol.