CELLULAR RESISTANCE TO VIRAL INFECTION

我们将继续沿着我们的研究开辟的充满希望的道路 弹状病毒的两个主要成分：核衣壳的分析 基因组及其聚合酶，以及病毒粒子的被膜(膜)和其 激活的组件。我们正在调查病毒转录和病毒转录的控制 复制。我们将继续研究VS病毒的机制 中断细胞RNA的合成，从细胞核运输，加工， 多聚腺苷和多聚核糖体的形成。我们正在继续研究 VS病毒温敏突变体及其致病性和致病性 对小鼠的免疫原性。我们正试图确定脂质之间的联系 通过亲和标记获得基质(M)蛋白。合作研究将 继续与生化系的同事们一起学习。我们将登上一辆 对病毒膜结构和功能的正面攻击 精细的化学和生物物理技术。这些研究应该澄清 病毒的膜-膜相互作用及病毒感染机制 萌芽中。

We shall continue on the promising paths opened up by our research into the analysis of the two principal components of rhabdoviruses: the nucleocapsid genome and its polymerase enzymes, and the virion envelope (membrane) and its active components. We are investigating controls of VS viral transcription and replication. We will continue to examine the mechanisms by which VS virus interrupts cellular RNA synthesis, transport from the nucleus, processing, polyadenylation and polyribosome formation. We are continuing studies on temperature-sensitive mutants of VS virus and their pathogenicity and immunogenicity for mice. We are attempting to determine the lipid association of the matrix (M) protein by affinity labeling. Collaborative studies will continue with colleagues in the Biochemistry Department. We shall mount a frontal attack on the structure and function of viral membranes by use of refined chemical and biophysical techniques. These studies should elucidate the mechanisms of virus infection by membrane-membrane interaction and of virus budding.