AGING-SPECIFIC GENE EXPRESSION IN DROSOPHILA

为了开始了解衰老的生物学，我们建议识别基因 它们在衰老过程中差异表达，并决定了 这一规定的机制。热休克蛋白70(Hsp70)基因 在衰老过程中被发现在肌肉中被诱导，并使用转基因 苍蝇，这种诱导被发现需要之前未被识别的 启动子元素。这些相同的新元素也可能介导hsp70O 氧自由基的诱导作用。我们建议详细分析 调节衰老特异性的启动子序列和反式作用因子 Hsp7O的表达及其与氧自由基的可能关系 制作。 为了识别更多具有年龄特异性表达模式的基因，我们 分析了600个含有基因工程转座子的果蝇品系 被称为“增强子陷阱”，充当表达的记者。 基因的模式。12个品系表现出不同的老化和组织特异性 表情模式。其中几个增强子确定的基因- 将识别、克隆和分析陷阱，目标是 理解基因的组织特异性变化的机制 在衰老过程中的表达。

To begin to understand the biology of aging, we propose to identify genes which are differentially expressed during aging, and determine the mechanisms for this regulation. The heat shock protein 70 (hsp7O) gene was found to be induced in muscle during aging, and using transgenic flies, this induction was found to require previously unidentified promoter elements. These same novel elements may also mediate hsp7O induction by oxygen radicals. We propose to analyze in detail the promoter sequences and trans-acting factors which regulate aging-specific hsp7O expression, and their possible relationship to oxygen radical production. To identify additional genes with aging-specific expression patterns, we have analyzed 600 fly lines containing genetically engineered transposons called "enhancer-traps", which act as reporters for the expression patterns of genes. 12 lines exhibit various aging- and tissue-specific expression patterns. The genes identified by several of these enhancer- traps will be identified, cloned and analyzed, with the goal of understanding the mechanisms for tissue-specific changes in gene expression during aging.