PATHOBIOLOGY OF RETROVIRUSES

描述：(改编自申请者的摘要)调查员有两个 明确的目标。第一是研究遗传多样性和系统发育。 在来自非洲非人类灵长类动物的自然产生的SIV中。这个 作者将尝试更严格地定义病毒进化和 SIVagm(非洲绿猴SIV)病毒的分化与 它们的寄主物种。来自西非的SIVagm(SAB)全长克隆 来自Tantalus猴子的绿猴子和SIVagm(棕褐色)将被测序并 与其他两种非洲绿猴(马尾猴和黑猩猩)相比 格里夫茨)。其他SIVagm(GRI)和SIVabm(VER)病毒将 分析以确定来自马鞭草的物种特异性SIVagm病毒， 格里韦特猴、沙贝猴和坦塔卢斯猴进化和分化一致 与它们各自的寄主物种。此外，作者还将展开 基因研究将来自其他猴子物种的病毒纳入 天顶猴属，包括帕塔斯和哈姆林的猴子。第二 研究人员的目标是定义和比较病毒和宿主 自然发生的SIV发病的决定因素。目的 这一目标是为了定义病毒和宿主因素在 确定对疾病的天然抵抗力。某些SIVagm菌株可能 比其他人更具致病性。更多致病菌株的研究 SIVagm菌株可能导致特定决定因素的鉴定 直接参与发病机制。分子克隆和生物克隆 从患有SIV相关异常的绿猴中衍生出来的将进一步 在绿猴动物模型上进行研究。

DESCRIPTION: (Adapted from applicant's abstract) The investigator has two specific aims. The first is to study genetic diversity and phylogeny among naturally-occurring SIVs from African non-human primates. The author will attempt to more rigorously define viral evolution and divergence of SIVagm (SIV in African Green Monkeys) viruses in relation to their host species. Full-length clones of SIVagm(sab) from West African green monkeys and SIVagm(tan) from tantalus monkeys will be sequenced and compared with two other species of African green monkeys (vervet and grivets). Additional SIVagm (gri) and SIVabm(ver) viruses will be analyzed to determine whether species-specific SIVagm viruses from vervet, grivet, sabaeus and tantalus monkeys have evolved and diverged coincident with their respective host species. In addition, the author will expand genetic studies to include viruses from other monkey species within the Cercopithecus genus, including Patas and Hamlyn's monkeys. The second goal of the investigator is to define and compare viral and host determinants of pathogenesis for naturally-occurring SIVs. The purpose of this aim is to define viral and host factors which play a role in determining natural resistance to disease. Certain strains of SIVagm may be more pathogenic than others. The study of more pathogenic strains of SIVagm strains may lead to the identification of specific determinants directly involved in pathogenesis. Both molecular and biologic clones derived from green monkeys with SIV-related abnormalities will be further studied in the green monkey animal model.