PATHOBIOLOGY OF RETROVIRUSES

描述：（改编自申请人摘要）研究者有两个 具体目标。 首先是研究遗传多样性和遗传学 在非洲非人类灵长类动物中自然发生的SIV。 的 作者将试图更严格地定义病毒的进化， SIVagm（非洲绿色猴中的SIV）病毒与 宿主物种。 来自西非的SIVagm（sab）全长克隆 将对来自tantalus猴的绿色猴和SIVagm（棕褐色）进行测序， 与其他两种非洲绿色猴（长尾猴和 grivets）。 其他SIVagm（gri）和SIVabm（ver）病毒将被 分析以确定是否来自长尾猴的物种特异性SIVagm病毒， grivet，sabaeus和tantalus猴子进化和分歧一致 与它们各自的宿主物种。 此外，作者还将扩大 基因研究，包括其他猴子物种的病毒， Cercopithecus属，包括Patas和Hamlyn的猴子。 第二 研究者的目标是定义和比较病毒和宿主 自然发生的SIV的致病决定因素。 的目的 这一目的是确定病毒和宿主因子， 决定了对疾病的天然抵抗力。 某些SIVagm菌株可能 比其他人致病性更强。 对致病性更强的菌株的研究 SIVagm株可能导致识别特定的决定簇 直接参与发病机制。 分子克隆和生物克隆 来自SIV相关异常的绿色猴的细胞将进一步 在绿色猴动物模型中研究。

DESCRIPTION: (Adapted from applicant's abstract) The investigator has two specific aims. The first is to study genetic diversity and phylogeny among naturally-occurring SIVs from African non-human primates. The author will attempt to more rigorously define viral evolution and divergence of SIVagm (SIV in African Green Monkeys) viruses in relation to their host species. Full-length clones of SIVagm(sab) from West African green monkeys and SIVagm(tan) from tantalus monkeys will be sequenced and compared with two other species of African green monkeys (vervet and grivets). Additional SIVagm (gri) and SIVabm(ver) viruses will be analyzed to determine whether species-specific SIVagm viruses from vervet, grivet, sabaeus and tantalus monkeys have evolved and diverged coincident with their respective host species. In addition, the author will expand genetic studies to include viruses from other monkey species within the Cercopithecus genus, including Patas and Hamlyn's monkeys. The second goal of the investigator is to define and compare viral and host determinants of pathogenesis for naturally-occurring SIVs. The purpose of this aim is to define viral and host factors which play a role in determining natural resistance to disease. Certain strains of SIVagm may be more pathogenic than others. The study of more pathogenic strains of SIVagm strains may lead to the identification of specific determinants directly involved in pathogenesis. Both molecular and biologic clones derived from green monkeys with SIV-related abnormalities will be further studied in the green monkey animal model.