INHIBITION OF INFLAMMATORY PROCESSES

这项研究的目的是确定病毒抑制的机制， 炎症 这一信息应能增进我们对两者的了解 特异性炎症反应的调节，以及 这些反应的特定介质。 牛痘病毒是一种含有DNA的病毒，编码约200个基因， 产品，能够抑制哺乳动物的炎症反应 防止病毒感染。 由于该属性，它提供了一个独特的 用于研究炎症过程的系统。 该系统具有 已经使我们能够鉴定出两种新的炎症抑制剂， 介质：IL-1 β成熟酶的抑制剂和分泌的 TNFa结合蛋白。 这两种病毒蛋白不足以 抑制炎症反应，即使两个细胞因子的目标， 抑制剂是炎症的关键介质。 两种抑制剂 必须与其他病毒蛋白一起作用，以抑制多种炎症反应， 流程. 这项研究的具体目标是： 1.为了鉴定有助于抑制的每种病毒蛋白， 炎症反应。 2.为了表征抗炎蛋白及其作用过程， 禁止。 3.为了确定病毒蛋白抑制 炎症过程。 标准的遗传和生化程序将用于实现这些 目的，利用这种生物的特殊性质， 系统，包括这些基因组成的容易程度， 病毒是可以被改造的。 了解这种有效抑制病毒的机制 炎症反应的研究将有助于开发新的方法 来控制急性和慢性炎症。

The goal of this study is to identify mechanisms of viral inhibition of inflammation. This information should enhance our understanding of both the regulation of specific inflammatory responses, and the role of specific mediators of these responses. Cowpox virus, a DNA-containing virus encoding about two hundred gene products, is capable of inhibiting mammalian inflammatory responses against virus infection. Because of this property, it provides a unique system for the investigation of inflammatory processes. This system has already enabled us to identify two novel inhibitors of inflammatory mediators: an inhibitor of the IL-1beta maturase, and a secreted TNFalpha-binding protein. These two viral proteins are not sufficient to inhibit inflammatory responses, even though the two cytokine targets of the inhibitors are pivotal mediators of inflammation. The two inhibitors must act with other viral proteins to inhibit the multiple inflammatory processes. The specific aims of this study are: 1. To identify each of the viral proteins contributing to the inhibition of inflammatory responses. 2. To characterize the anti-inflammatory proteins and the processes they inhibit. 3. To determine the mechanisms by which the viral proteins inhibit inflammatory processes. Standard genetic and biochemical procedures will be used to achieve these aims, taking advantage of the special properties of this biological system, including the ease with which the genetic composition of these viruses can be modified. Knowledge of the mechanisms involved in this effective viral inhibition of inflammatory responses should assist the development of novel methods to manage both acute and chronic inflammation.