HIV-1 VPR INTERACTION WITH A HOST CELLULAR PROTEIN

描述(改编自调查员摘要)：中心焦点 此应用的目的是克隆vpr相互作用蛋白的cdna。 (RIP)，并探讨VPR和VPR的生化作用机制。 HIV复制过程中的RIP。具体地说，RIP将从 细胞提取物和微测序，因此寡核苷酸探针可以 设计用于筛选cdna文库。然后，RIP的表达和激活 将对不同类型的原代人体血细胞进行检测。 随后，VPR和RIP在艾滋病毒复制过程中的作用将是 通过建立表达VPR或RIP的细胞系进行检查，通过 反义抑制内源性RIP的表达 寡核苷酸，并通过使用体内和体外系统 检测VPR和RIP对HIV早期转录的影响 普罗鲁斯。作者希望从这些研究中获得关于以下方面的信息 辅助基因如何调控HIV在宿主中的复制 VPR和蜂窝目标RIP。

DESCRIPTION (Adapted from investigator's abstract): The central focus of this application is to clone the cDNA for the Vpr-interacting protein (RIP) and to explore the biochemical mechanism of function of Vpr and RIP during HIV replication. Specifically, RIP will be purified from cellular extracts and micro-sequenced so oligonucleotide probes can be designed to screen cDNA libraries. Then, RIP expression and activation in different types of primary human blood cells will be examined. Subsequently, the role of VPR and RIP during HIV replication will be examined by establishing Vpr or RIP-expressing cell lines, by suppression of endogenous RIP expression with anti-sense oligonucleotides, and by using in vivo as well as in vitro systems to measure effects of Vpr and RIP on the early transcription of the HIV provirus. From these studies the author expects to gain information on how HIV replication in the host may be regulated by the accessory gene vpr and the cellular target RIP.