ROLES OF LAMININ AND ENTACTIN IN CELL ADHESION

细胞外基质对血管的正常功能起着至关重要的作用。 复杂的有机体。它与器官发生、细胞 分化、转移、神经引导、伤口愈合和 具有保护功能。的专用片状结构。 细胞外基质，即基底膜，位于各种组织的下方，是 与上面细胞的质膜密切接触。这个 这种结构的主要成分是层粘连蛋白、IV型胶原、肝素 硫酸盐蛋白多糖和肌动蛋白。这些化合物的分子结构 分子在很大程度上是由重组DNA方法和 每个分子的生物活性正在被阐明。 跨膜受体已被鉴定和表征为 层粘连蛋白和IV型胶原。这些跨膜受体被认为 负责将信息从细胞外传输到 细胞内的隔间，从而引发特定的细胞 回应。肌动蛋白的生物学特性，包括促进 细胞黏附、趋化、吞噬和中性粒细胞脱颗粒 直到现在才被发现。此外，entactin似乎是 在通过细胞外基质的相互作用组织细胞外基质方面很重要 含层粘连蛋白、IV型胶原和纤维连接蛋白。它的目的是 本项目旨在扩大对entactin的研究，并在体外进行相关研究 具有体内生物学功能的观察。将要进行的实验是 执行包括(A)映射功能域(B)隔离和 跨膜和其他受体的特性(C)其受体的分析 在眼睛的形态发生和发育中的作用和(D)探索 它在止血和伤口愈合中的作用。一种组合 将使用免疫学、生化和遗传方法来解决 这些问题。希望所取得的成果将有助于 我们在分子水平上对基膜功能的理解 发育正常，处于病理状态。

The extracellular matrix plays a vital role in the normal function of complex organisms. It is implicated in organogenesis, cell differentiation, metastasis, neuronal guidance, wound healing and protective functions. A specialized sheet-like structure of the extracellular matrix, the basal lamina, underlies various tissues and is in intimate contact with the plasma membrane of the overlying cells. The major components of this structure are laminin, type IV collagen, heparan sulfate proteoglycan and entactin. The molecular structures of these molecules have been largely determined by recombinant DNA approaches and the biological activity of each molecule is being elucidated. Transmembrane receptors have been identified and characterized for laminin and type IV collagen. These transmembrane receptors are believed to be responsible for transmitting information from the extracellular to the intracellular compartment and thereby eliciting specific cellular responses. The biological properties of entactin which include promotion of cell attachment, chemotaxis, phagocytosis and neutrophil degranulation are only now being uncovered. Furthermore , entactin appears to be important in organizing the extracellular matrix through its interactions with laminin, type IV collagen and fibronectin. It is the purpose of this project to extend the studies on entactin and to correlate in vitro observations with in vivo biological functions. The experiments to be performed include (a) mapping the functional domains (b) isolation and characterization of transmembrane and other receptors (c) analysis of its role in the morphogenesis and development of the eye and (d) exploring its function in hemostasis and wound healing. A combination of immunological, biochemical and genetic approaches will be used to solve these problems. It is hoped that the results obtained will contribute to our understanding of basal lamina function at the molecular level in normal development and in pathological conditions.