GENETIC ANALYSIS OF HEMOPOIETIC HISTOCOMPATIBILITY

描述：（改编自申请人的摘要）长期 本项目的目的是了解遗传机制（S） 这是对造血同种异体移植的天然抵抗的基础。 作为初始 为实现上述目标，提出了三个具体目标： 1)以确定Hh-1基因座在H-2-B和H-2-d中的位置 限制性片段长度多态性（RFLP） 在一组H-2S/D重组单倍型中，2）鉴定和分离 Hh-1基因，使用人和鼠H-2 S/D间隔和H-2-B探针 和H-2-d粘粒文库，以及3）启动对 基因识别。 拟议的方法应提供新的见解 研究自然抗性的遗传机制， NK细胞的识别功能。 此外，这些研究应该有助于 鉴定人类同源基因，提高临床骨的前景 骨髓移植，并可能发展NK细胞介导的癌症 免疫疗法

DESCRIPTION: (Adapted from the applicant's abstract) The long-term objective of this project is to understand the genetic mechanism(s) underlying the natural resistance to hemopoietic allografts. As an initial approach towards the stated objective, three specific aims are proposed: 1) to determine the location of the Hh-1 locus in the H-2-b and H-2-d chromosomes by determining restriction fragment length polymorphism (RFLP) in a panel of H-2S/D recombinant haplotypes, 2) to identify and isolate the Hh-1 genes, using human and murine probes for the H-2S/D interval and H-2-b and H-2-d cosmid libraries, and 3) to initiate the characterization of the genes thus identified. The proposed approach should provide new insight into the genetic mechanism underlying natural resistance and the recognition function of NK cells. In addition, these studies should help identify homologous genes in man, improve the prospect of clinical bone marrow transplantation, and possibly develop NK cell-mediated cancer immunotherapy.