PCR DEVELOPMENT AND LOCALIZATION OF CRI RFLP PROBES

这项研究的目标是开发符合pcr的微卫星。 来自大约110个RFLP探针的标记，并定位大约 320个基因定位到染色体区域的探针。新的 微卫星标记将被放置在遗传图谱上，以确保 它们与原始的RFLP探针紧密相连。 标记的RFLP探针是研究以下领域的重要工具 遗传病基因定位及杂合性丢失研究 肿瘤细胞。将RFLP探针转化为可聚合酶链式反应的优势 微卫星重复标记是两个重复标记。首先，标记将是 信息量大得多。其次，基于聚合酶链式反应的基因分型方法是 与目前使用的基因组Southern相比有显著改进 杂交法。这些PCR标记也可以作为STSS应用于 构建人类染色体的物理图谱，用于诊断 遗传性疾病和肿瘤，以及身份测试。 遗传图谱和细胞遗传学图谱之间的关联将有助于 定位疾病基因和涉及癌症的基因。这些探头 被定位到医学上重要的区域可以用来开发 荧光原位杂交(FISH)诊断试验。在……里面 此外，确定物理位置将大大增加 我们的许多问题证明了探头的适销性 调查客服已收到。它还将使我们能够追查 展望未来的临床市场。

The goal of this research is to develop PCR-amenable microsatellite markers from approximately 110 RFLP probes and to localize approximately 320 genetically mapped probes to chromosomal regions. The new microsatellite markers will be placed on the genetic map to ensure that they are tightly linked to the original RFLP probes. Mapped RFLP probes are an important tool for research in areas such as mapping genetic disease genes and studying loss of heterozygosity in tumor cells. The advantage of converting RFLP probes into PCR-amenable microsatellite repeat markers is two fold. First, the markers will be much more informative. Second, PCR-based genotyping methods are a significant improvement over currently used genomic Southern hybridization methods. The PCR markers can also be used as STSs for constructing physical maps for human chromosomes, for diagnosis of genetic diseases and tumors, and for identity testing. Correlation between the genetic and cytogenetic maps will facilitate localizing disease genes and genes involved in cancer. The probes that are localized to medically important regions can be used to develop fluorescence in situ hybridization (FISH)-based diagnostic tests. In addition, determining the physical location will greatly increase the marketability of probes as evidenced by the numerous questions that our Probe customer Service has received. It will also allow us to pursue the clinical market in the future.