5D Imaging Flow Cytometry for in vivo Quantification of Biological Fluids

在亚细胞和分子水平上快速准确地定量活生物流体特性是生物流体科学的基石。大多数生物流体装置依赖于体外流式细胞仪（FC）对从体内取出的生物流体进行定量。FC面临着生物降解和体积小等诸多问题。在这个项目中，我们将设计第一台体内5D成像流式细胞仪（5D IFC），能够连续评估活体小鼠的潜在全血容量，而无需将液体排出体外。该项目代表了一项超越现有流式细胞仪的重大进步，并克服了最先进的激光扫描成像设备的工程限制。

Rapid and accurate quantification of live biological fluid properties at sub-cellular and molecular levels forms the bedrock of biofluidic sciences. Majority of the biofluidic devices rely on quantifying biological fluids after its removal from the body in an in vitro Flow Cytometer (FC). FC faces many caveats i.e. biological degradation and small volume etc. In this project, we shall engineer the first in vivo 5D imaging flow cytometer (5D IFC) capable of continuous assessment of potentially entire blood volume in a living mice without removing fluid out of the body. The project represents a major advancement beyond any existing flow cytometer and overcome the engineering limits of state-of-art laser scanning imaging devices.