VESTIBULAR REFLEX RECOVERY AFTER NEUROTOLOGICAL SURGERY

持续性和致残性眩晕和平衡失调的原因 前庭损伤是未知的。 拟议研究的目的是 改善平衡障碍患者的治疗， 关于前庭系统的反应的新信息 耳神经外科 这个项目的具体目标是，首先，检验假设， 急性前庭损伤的部位，是否仅限于前庭 神经上皮和节前前庭神经或涉及后 前庭神经节损伤，影响前庭功能恢复 反射 第二个具体目标是测试时间进程是否 前庭损伤，急性与渐进性，影响恢复 前庭反射 第三个具体目标是开始调查 通过研究急性和长期的细胞补偿机制， 中枢前庭核内的跨神经变化。 这些特定的目的将在前庭的猫动物模型中进行测试。 耳神经外科 前两个具体目标将审查复苏情况 前庭反射的症状 为了实现这些目标，我们 将检查水平前庭眼反射（HVOR），耳石- 眼反射（OOR）、前庭-结肠反射（VCR）和凝视 在联合眼头运动期间的稳定性。 最终的具体目标 包括对中枢前庭神经系统的定量组织病理学分析， 利用银变性染色在这些相同动物中的细胞核， 细胞形态计量学和免疫细胞化学检测星形胶质细胞特异性 胶质细胞酸性蛋白（GFAP）。 这项研究将增加我们对人类的反应的理解。 前庭系统消融前庭病变。 其意义 研究是，从拟议的研究数据将提供基本的 为临床医生提供科学信息，使他们能够选择 切除手术，产生最大的功能恢复。

The cause of persistent and disabling vertigo and disequilibrium following vestibular injury is not known. The objective of the proposed research is to improve the treatment of patients with balance disorders by providing new information about the response of the vestibular system to neurotological surgery. The specific aims of this project are first, to test the hypothesis that the site of acute vestibular injury, whether limited to the vestibular neuroepithelium and pre-ganglionic vestibular nerve or involving post- ganglionic vestibular nerve injury, influences the recovery of vestibular reflexes. The second specific aim is to test whether the time course of vestibular injury, acute versus gradual, influences the recovery of vestibular reflexes. The third specific aim is to begin to investigate the mechanisms of compensation by studying acute and long-term cellular and transneural changes within the central vestibular nuclei. These specific aims will be tested in a cat animal model of vestibular neurotological surgery. The first two specific aims will examine recovery of vestibular reflexes following surgery. To accomplish these aims, we will examine the horizontal vestibulo-ocular reflex (HVOR), the otolith- ocular reflex (OOR), the vestibulo-collic reflex (VCR), and gaze stabilization during combined eye-head movements. The final specific aim involves a quantitative histopathologic analysis of the central vestibular nuclei in these same animals utilizing silver degeneration stains, cytomorphometrics, and immunocytochemistry for the astroglial specific protein glial fibrillary acidic protein (GFAP). This research will increase our understanding of the response of the vestibular system to ablative vestibular lesions. The significance of this research is that the data from the proposed studies will provide basic scientific information to clinicians which may enable them to select ablative procedures which yield the greatest functional recovery.