MICROLITHOGRAPHIC ARRAYS FOR DNA FRACTIONATION

我们已经利用国家纳米制造设施的资源， 微光刻准二维障碍阵列。这些设备是 用于探测和处理这些生物聚合物 微影光刻表面。利用这笔赠款的支持，我们建议 购买仪器进行高灵敏的实时荧光 超大单体运动的成像观测与分析 聚合物分子通过这些阵列。这些措施的直接目的是 实验是在染色体长度尺度上分离DNA聚合物 在一个高度可复制的环境中。在学习中获得的经验 如何通过合成微影技术移动超长DNA分子 在该项目的后期阶段，将利用表面来对齐 染色体长度的DNA分子，并分析DNA序列和 其他与生物相关的特性。

We have built, using resources of the National Nanofabrication Facility, microlithographic quasi two-dimensional obstacle arrays. These devices are being used to both probe and process biological polymers on these microlithographic surfaces. Using support from this grant, we propose to acquire instrumentation to do highly sensitive realtime fluorescence imaging to observe and analyze the motions of extremely large single polymer molecules through these arrays. The immediate aim of these experiments is to fractionate DNA polymers at the chromosome length scale in a highly reproducible environment. The experience gained in learning how to move very long DNA molecules through synthetic microlithographic surfaces will be exploited in the latter phase of this project to align chromosomal length DNA molecules and analyze both the DNA sequence and other biologically relevant properties.