TRIGEMINAL BRAINSTEM NOCICEPTIVE NEURONS IN VITRO

这项提案的长期目标是：a)确定 大脑皮层I层神经元的电膜特性和形态 三叉神经感觉核复合体尾侧亚核，b)至 确定这些神经元对电刺激的突触反应 三叉神经传入，c)检查选择性干扰 抑制性神经递质受体改变大鼠突触反应 I层神经元。 为了实现这些目标，各种细胞内记录和 将使用组织化学技术来研究膜的性质， 水平方向三叉神经神经元的突触反应和形态 豚鼠脑干的切片制备。有待检验的假设 包括：1)高频刺激三叉神经传入产生 尾侧亚核内神经肽介导的慢兴奋性电位 I层神经元2)P物质介导的慢兴奋电位 是由NK1速激肽受体亚型引起的阵发性 阻断神经元后，可在I层神经元诱发放电 GABA(Beta)-受体亚型。 建议的研究将作为深入分析 三叉神经系统中的信号传输。所获得的知识可以 为后续的研究工作提供科学依据 对肌肉面部等衰弱状况的认识和治疗 具有重要临床意义的疼痛和三叉神经痛 在牙科。

The long-term objectives of this proposal are: a) to characterize the electrical membrane properties and morphology of lamina I neurons in the subnucleus caudalis of the trigeminal sensory nuclear complex, b) to determine the synaptic responses of these neurons to activation of trigeminal afferent inputs, c) to examine how selective interference with inhibitory neurotransmitter receptors alters the synaptic responses of lamina I neurons. To achieve these objectives, various intracellular recording and histochemical techniques will be used to study the membrane properties, synaptic responses and morphology of trigeminal neurons in the horizontal slice preparation of the guinea pig brainstem. Hypotheses to be tested include: 1) high frequency stimulation of trigeminal afferents produces slow, neuropeptide-mediated excitatory potentials in subnucleus caudalis lamina I neurons 2) the substance P-mediated slow excitatory potentials are evoked via the NK1 tachykinin receptor subtype 3) paroxysmal discharges may be evoked in lamina I neurons following blockade of the GABA(Beta)-receptor subtype. The proposed studies will serve as a basis for the in depth analysis of signal transmission in the trigeminal system. The obtained knowledge can subsequently be used to provide a scientific basis for the complete understanding and treatment of debilitating conditions such as myofacial pain and trigeminal neuralgia which are of major clinical significance in dentistry.