Cost Effective Dual Laser System for Nonlinear CARS Microscopy

生物医学研究是一个巨大的高价值市场，荧光显微镜是最常用和最强大的工具之一。荧光成像技术需要用染料标记被研究的区域，这通常会扰乱细胞功能，并释放出任何典型的毒素。相反，相干反斯托克斯拉曼散射(CARS)显微镜使研究人员能够利用非线性显微镜的高3D分辨率能力，同时避免光毒性问题。此外，它还可以提供分子特有的信息。CARS在生物学中得到了广泛的应用，它被用于DNA分析和癌症检测。这项研究的主要目的是研究在CARS显微镜中使用两个工作在不同波长的脉冲SDL激光器的可行性。

Biomedical research is a large high value market and fluorescence microscopy are some of the most common and powerful tools used. Fluorescence imaging techniques require the region to be studied to be labelled with a dye, this often disrupts cell function and any typically releases toxins. In contrast Coherent anti-Stokes Raman Scattering (CARS) microscopy enables the researcher to harness the high 3D resolution capability of nonlinear microscopy while avoiding the photo-toxicity issue. In addition it can provide molecule specific information. CARS has found diverse applications in biology, and it is employed in DNA analysis and cancer detection. The primary aim of the study is to look at the feasibility of using two pulsed SDL lasers operating at dissimilar wavelengths for use in a CARS microscope.