Engineering A Novel Bacterial Host for Biobutanol Production

我们的目标是开发一种新的细菌宿主，用于从可再生原料生产1-丁醇。该策略的重点是梭菌物种（巴氏梭菌）的修改。这种微生物具有许多理想的特征，使其成为有吸引力的发酵宿主（快速生长速率、鲁棒性和良好的丁醇耐受性），但受到有限的底物范围、相对较差的丁醇产率的影响，并且非常难以遗传操纵。在这个项目中，我们将为梭菌部署先进的分子生物学工具，为淀粉代谢和丁醇生产引入合成代谢途径。竞争的副产品将被淘汰。可交付物将是一种新的工程菌株C。巴氏杆菌，其以高产率将淀粉发酵成丁醇。

We aim to develp a novel bacterial host for the production of 1-butanol from renewable feedstocks. The strategy focuses on the modification of a clostridia species (Clostridium pasteurianum). This microbe has many desirable features that make it an attractive fermentation host (fast growth rates, robustness and good butanol tolerance) but suffers from a limited substrate range, relatively poor butanol yields and is notriously difficult to genetically manipulate. In this project, we will deploy advanced molecular biology tools for Clostridia to introduce synthetic metabolic pathways for starch metabolism and also for butanol production. Competing byproducts will be knocked out. The deliverable will be a novel engineered strain C. pasteurianum that ferments starch to butanol in high yield.