ELECTROPHYSIOLOGY OF CILIARY BODY TRANSPORT PROCESSES

该提案的长期目标是研究细胞 与睫状体产生水状体有关的机制 上皮具体目标是体外电生理学研究， 睫状上皮的分离制备物上的运输过程。 这些实验将提供关于正常生理学的信息， 机制和药理学诱导的改变参与的活性 水状体的产生。减少房水形成， 药物治疗是眼内压升高的常见治疗方法， 与青光眼相关的压力。关于细胞机制的知识 负责水状体生产将提供深入了解 正常的房水形成率和可能的药理学 降低房水生成速率的机制。 安装分离的睫状体制备物用于测量 跨上皮电参数（电位差、短路 流动）、净流体输送和单向通量。这些研究 代表组织中主动运输和流体流动的巩固 准备保持正常的解剖关系之间的 睫状突的双层上皮。常规微量移液器 用于测量膜电位的穿刺研究在 结合经上皮测量。这些研究提供 关于个体睫状上皮细胞与离子关系的信息 运动和运输系统。此外，细胞内离子电渗疗法 进行荧光染料荧光黄的注射以研究间隙 为细胞间通讯提供途径的连接（细胞- 细胞偶联），从而允许细胞作为合胞体发挥功能。 电生理学研究用于研究以下类别 （i）卤代麻醉剂，（ii）肾上腺素能激动剂， 神经肽Y与肾上腺素能激动剂的相互作用，（iii） 巴比妥酸盐和（iv）叠氮化钠。这些实验将提供 关于积极生产水的机制的资料 可能导致新的和独特的抗青光眼药物， 抑制房水产生中的不同顺序步骤。

Long-term objective of this proposal is the investigation of cellular mechanisms relating to the production of aqueous humor by the ciliary epithelium. Specific aims are the in vitro electrophysiologic study of transport processes on isolated preparations of the ciliary epithelium. These experiments will furnish information on normal physiological mechanisms and pharmacological-induced alterations involved in the active production of aqueous humor. Reduction of aqueous humor formation by medical therapy is a frequent treatment of the elevated intraocular pressure associated with glaucoma. Knowledge regarding cellular mechanisms responsible for aqueous humor production will provide insights into the normal rate of aqueous humor formation and possible pharmacologic mechanisms for the reduction in the rate of aqueous humor production. Isolated ciliary body preparations are mounted for measurement of transepithelial electrical parameters (potential difference, short-circuit current), net fluid transport, and unidirectional fluxes. These studies represent a consolidation of active transport and fluid flow in a tissue preparation maintaining the normal anatomic relationships between the double epithelial layers of the ciliary processes. Conventional micropipet impalement studies for measurement of membrane potential are performed in conjunction with the transepithelial measurements. These studies provide information on individual ciliary epithelial cells in relationship to ion movements and transport systems. In addition, intracellular iontophoretic injection of the fluorescent dye Lucifer Yellow is performed to study gap junctions which provide a pathway for intercellular communication (cell- cell coupling), thereby allowing cells to function as a syncytium. Electrophysiologic investigations are used to study the following classes of drugs: (i) halogenated anesthetic agents, (ii) adrenergic agonists and the interaction of neuropeptide Y with the adrenergic agonists, (iii) barbiturates, and (iv) sodium azide. These experiments will furnish information on mechanisms involved in the active production of aqueous humor and may result in new and unique antiglaucoma agents designed to inhibit the different sequential steps in aqueous humor production.