BIOCHEMICAL AND PHARMACOLOGICAL INVESTIGATIONS

该方案的目标是扩大和加强 佛罗里达A&M大学（FAMU）研究人员在生物医学领域的能力 研究，为本科生提供“动手”经验 科学研究的基础知识，并激励他们追求 生物医学研究职业以及培养研究生在 生物医学科学的最新研究， 在药理学、药物化学、生物化学、分子生物学和 毒理学 拟议的研究计划将涉及使用 实验室动物 这些子项目有以下主题：（1） 环境和代谢性外源物的电化学研究 （2）α-辅肌动蛋白基因的序列分析及其在细胞内的表达调控 甲状腺激素;（3）硒化合物与镉的肺毒性; (4)秋水仙碱和紫杉醇类似物作为微管蛋白探针的研究（5）生物学 南极隐石内真菌产生的活性物质（6） 过度使用的骨骼肌中的结缔组织变化;（7） 肥大细胞的非免疫激活-分泌偶联;（8）疾病 糖尿病中甲硫氨酸水平的研究;（9）铬酸盐的分子机制 （10）镉的衍生物 皮质类固醇代谢（11）钙在细胞毒性中的作用 （12）使用差示扫描量热法研究 载脂蛋白与胰脂肪酶（十三）合成与生物学 几种N-氨基四氢吡啶类化合物的活性测定（14） 三种胰岛素抵抗模型大鼠药物代谢的比较 (15)戊巴比妥反应动物模型的建立;（16）锌 镉诱导大鼠肝毒性的饮食改变;（17） 新生儿铅暴露对肾上腺皮质功能的影响（18） 时间生物素药物有益作用的神经化学基础。 分析程序包括光谱测定（例如， 紫外、红外、质子NMR、31-P NMR原子吸收）， 色谱工作（例如，TLC，GC，HPLC）和许多药理学 使用闪烁计数器的测定将在这些 调查事务所 研究生和本科生都将 参与实验的计划和执行。

The objective of this program is to expand and strengthen the capabilities of Florida A&M University (FAMU) investigators in biomedical research, to provide undergraduate students with "hands-on" experience in the fundamentals of scientific research and to motivate them to pursue biomedical research careers as well as to train graduate students in state of the art research in the biomedical sciences with concentration in pharmacology, medicinal chemistry, biochemistry, molecular biology and toxicology. The proposed research program will involve studies using laboratory animals. The subprojects have the following topics: (1) An Electrochemical Study of Environmental and Metabolic Xenobiotic Oxidation; (2) Sequence Analysis of -Actinin Gene and Its Regulation by Thyroid Hormones; (3) Selenium Compounds and Cadmium Toxicity on Lungs; (4) Colchicine and Taxol Analogs as Probes of Tubulin; (5) Biologically Active Substances Produced by Antarctic Cryptoendolithic Fungi; (6) Connective Tissue changes in Overused Skeletal Muscles; (7) Nonimmunologic Activation-Secretion Coupling in Mast Cells; (8) disorder of Metenkephalin Levels in Diabetes; (9) Molecular Mechanisms of Chromate and Cadmium Toxicity in Mammalian Systems; (10) Derivatives of Corticosteroid Metabolites; (11) The Role of Calcium in the Cytotoxicity of Chlorophenols; (12) Use of Differential Scanning Calorimetry to Study Apolipoprotein and Pancreatic Lipase; (13) Synthesis and Biological Activity Determinations of Some N-Aminotetrahydropyridines; (14) Comparison of Three Insulin-Resistant Models on Drug Metabolism in Rats; (15) Developing an Animal Model for Response to Pentobarbital; (16) Zinc Dietary Alterations of Cadmium-Induced Hepatic Toxicity in Rats; (17) Neonatal Lead Exposure Effects on the Adrenal Cortex Function; (18) Neurochemical Basis of the Beneficial Effects of Chronobiotic Drugs. Analytical procedures including spectral determinations (e.g., ultraviolet, infrared, proton NMR, 31-P NMR atomic absorption), chromatographic work (e.g., TLC, GC, HPLC) and many pharmacological assays using scintillation counters will be routinely conducted in these investigations. Both the graduate and undergraduate students will be involved in the planning and execution of the experiments.