PLASMA CELLS IN THE LACRIMAL GLAND--IG SECRETION

描述(调查者摘要)：泪水，这是 泪腺不仅是角膜表面的润滑剂 也是对接触病毒的空气传播的抗原的主要防御 无血管角膜。泪液中的主要抗细菌/病毒制剂是 泪腺浆细胞分泌的免疫球蛋白。 泪头生理在细胞水平的阐明 对改善诸如“干燥”这样的条件至关重要 主要的焦点将是确定膜通道的作用。 受体介导的信使系统在调节 免疫球蛋白分泌。有证据支持这样的观点 浆细胞含有对神经递质有反应的受体， 这些相同的递质可以改变泪腺分泌的抗体。 腺体。鸡的哈德氏腺(泪腺)将成为系统 因为腺体有非常高密度的浆细胞，而且 鸟类泪腺分泌的主要免疫球蛋白是一种免疫球蛋白。 它被认为是被动地、原封不动地在 周围的皮质上皮(Mullock等人，1981)。这是 与大鼠泪腺明显不同，在那里分泌 免疫球蛋白(IgA)是二聚体，然后由分泌物分泌 上皮(Peppard和Montgomery，1987)。初步结果 表明有受体介导的机制能够调节 泪腺分泌免疫球蛋白及其受体的研究 被安置在腺体浆细胞的膜内。 电生理数据表明，膜电位(由 MAXI-K通道活性)是一种内在的向下调节 免疫球蛋白分泌。

DESCRIPTION (Investigator's Abstract): Tears, the secretory product of the lacrimal gland, serve not only as a lubricant of the corneal surface but also as a primary defense against airborne antigens that contact the avascular cornea. The major anti-bacterial/viral agents in tears are the immunoglobulins secreted by plasma cells of the lacrimal gland. Elucidation of the physiology of the lacrimal glans at the cellular level is of critical importance to the amelioration of conditions such as "dry eye." The major focus will be determination of the role membrane channels and receptor mediated messenger systems play in the modulation of immunoglobulin secretion. There is evidence to support the notion that plasma cells contain receptors responsive to neurotransmitters and that these same transmitters can alter antibody secretion by the lacrimal gland. The Harderian (lacrimal) gland of the chicken will be the system used because the gland has a very high density of plasma cells and the principle immunoglobulin secreted by the avian lacrimal gland is an IgG which is thought to diffuse passively and unmodified through the surrounding cortical epithelium (Mullock et al., 1981). This is distinctly different than the rat lacrimal gland where the secreted immunoglobulin (IgA) is dimerized and then secreted by the secretory epithelium (Peppard and Montgomery, 1987). The preliminary results indicate that there are receptor mediated mechanisms capable of modulating the secretory rate of IgG out of the lacrimal gland and that the receptors are housed within the membrane of the plasma cells of the gland. Electrophysiological data indicate that membrane potential (manipulated by maxi-K channel activity) is an intrinsic "down" regulator of immunoglobulin secretion.