NEW SENSORS BASED ON MOLECULAR RECOGNITION IN MONOLAYERS

这项提议的目标是开发一种通用的创作方法 用于生物分子的化学选择传感器。为此，分子 自组装单分子膜中的识别位点将由 通过以下方式在模板分子周围组装单层成分 分子间相互作用(离子或氢键)。后续 通过漂洗或裂解除去模板分子将留下 在识别部位后面用于模板的重吸收或非常 相似的分子。吸附分子的红外光谱和红外光谱 比较相似分子的吸附等温线将有所帮助 阐明识别位点特异性背后的原因。分子 然后将使用以下技术开发包含识别位置的传感器 厚度剪切模谐振器。避免非特定的干扰 谐振器的表面污染、频率和衰减 将在分子吸附过程中进行监测，因为衰减较小 可能被非特异性吸附改变的。通过结合识别 具有选择性检测的位置特异性，区分传感器应 被实现了。事实证明，这种传感器在诊断(监测)中是有用的 生物中间体)以及临床应用(监测 药物递送)。

The goal of this proposal is to develop a general method for the creation of chemically selective sensors for biomolecules. To this end, molecular recognition sites in self-assembled monolayers will be created by assembling monolayer constituents around template molecules via intermolecular interactions (ionic or hydrogen bonding). Subsequent removal of the template molecules by rinsing or cleavage will leave behind a recognition site for reabsorption of the template or very similar molecules. Infrared spectra of the adsorbed molecules and comparison of the adsorption isotherms of similar molecules will help elucidate the reasons behind recognition site specificities. Molecular sensors containing the recognition sites will then be developed using thickness shear mode resonators. To avoid interferences from nonspecific surface contamination, both frequency and attenuation of the resonator will be monitored during molecular adsorption because attenuation is less likely to be changed by nonspecific adsorption. By combining recognition site specificity with selective detection, discriminating sensors should be realized. Such sensors would prove useful in diagnostic (monitoring biological intermediates) as well as clinical applications (monitoring drug delivery).