Evaluating spectroscopic imaging of living skin equivalents as a new approach for topical formulations research

体外产品测试是外用应用和设备产品开发的重要组成部分，该项目旨在建立一种强大的非动物方法，作为一种新的方法，在临床前研究中更好地了解外用制剂的经皮吸收。该项目旨在开发药物在人体活体皮肤中的无标记成像(MSI)和受激拉曼光谱(SRS)。我们建议对这些药物成像技术进行第一次比较，并收集数据，以便对药物化合物在人和猪的体外皮肤以及HLSE中的渗透曲线进行强有力的评估。此外，我们将引入重大的新颖性和新的计量学，以支持HLSE和光谱成像的应用，以提供可靠和可移植的方案。该项目的最终目标是通过提供非动物模型和新的化学成像方案来支持3RS，这些方案经过局部配方测试的验证，以便在临床研究中能够可靠地预测安全性和有效性。

In vitro product testing is a vital part of product development for topical applications and devices and this project seeks to establish a powerful non-animal approach as a new method for providing a greater understanding of percutaneous absorption of topical formulations in pre-clinical studies. This project aims to develop label free imaging of drugs by mass spectrometry imaging (MSI) and stimulated Raman spectroscopy (SRS) in human living skin equivalents (HSLEs). We propose to undertake the first comparison of each of these techniques for imaging of drugs and to gather data to allow a robust evaluation of the penetration profiles of pharmaceutical compounds in human and pig ex vivo skin, and in an HLSE. In addition, we will introduce significant novelty and new metrology to support the application of the HLSEs and spectroscopic imaging to provide reliable and transferable protocols. The ultimate aim of this project is to support the 3Rs via the delivery of a non-animal model and novel chemical imaging protocols which are validated for topical formulation testing to allow reliable predictions of safety and efficacy in clinical studies.