Discovery and development of large/diverse user-friendly panels of novel biocatalysts

该项目旨在使用尖端的高通量专有生物信息学软件，称为“Filter-BLAST”，结合快速扩展的DNA数据库，在硅水平上有效准确地识别500个关键目标酶，代表了合成化学家酶工具箱中目前缺失的10个关键生物化学的多样性。先进/专有的高通量克隆技术（先前由Prozomix利用TSB基金开发），称为“GRASP”，将用于快速克隆所选择的目标基因和开发编码的生物催化剂，以经济有效地填充10个大型/新颖的关键酶筛选板，并将这些材料提供给生物催化社区，用于筛选感兴趣的特定个体生物催化剂。在发现符合其严格要求的酶之后，Prozomix的无动物、BSE-TSE认证形式的商业数量将用于在英国建立新的IB应用。

This project aims to use cutting-edge high-throughput proprietary bioinformatics software, called "Filter-BLAST", in conjunction with the ever rapidly expanding DNA databases, to efficiently and accurately identify at the in silico level 500 key target enzymes, representing a diversity of 10 key biochemistries currently missing from the synthetic chemists enzyme toolbox. Advanced/proprietary high-throughput cloning technology (previously developed by Prozomix using TSB funds), called "GRASP", will then be used to rapidly clone the target genes selected and develop the encoded biocatalysts to cost-effectively populate 10 large/novel screening panels of key enzymes, and provide these materials to the biocatalysis community for screening towards specific individual biocatalysts of interest. After discovering enzymes fitting their exacting requirements, commercial quantities in animal-free, BSE-TSE certified form from Prozomix will be used to establish the new IB applications in the UK.