ELECTRON TRANSFER PROTEINS

这份延期提案将提供有关控制 蛋白质-蛋白质电子的反应速率和反应专一性 转移反应。这样的反应在控制中至关重要 电子传递/氧化磷酸化过程中的生物能量流动 在许多代谢反应中也起着重要的作用。 我们将监测细胞色素C/细胞色素C过氧化物酶的反应动力学。 复合体，Cytb5：血红蛋白(肌红蛋白)复合体，以及Cytc：Cytc1 复杂，使用我们实验室开发的各种方法，具有特定的 重点是光化学电子转移。 因此，将进行结构研究，以了解 这些电子转移蛋白的结合和识别性质 并探索这种结构的动力学行为。 蛋白质复合体。已经为这种研究开发了两种新的方法， 分别侧重于核磁共振和能量转移技术。

This renewal proposal will provide information on the factors which control reaction rates and reaction specificity in protein-protein electron transfer reactions. Such reactions are centrally important in controlling biological energy flow in the electron transport/oxidative phosphorylation system, and also play an important role in many metabolic reactions. We will monitor the kinetics of reactions in the cytc/cyt c peroxidase complex, the cyt b5: hemoglobin (myoglobin) complexes, and the cytc:cyt c1 complex, using a variety of methods developed in our lab with a particular focus on photochemical electron transfer. Concomittantly, structural studies will be pursued to learn about the nature of binding and recognition in these electron transfer protein complexes, and to probe the dynamic behavior of the structure of such protein complexes. Two novel methods have been developed for such studies, focusing on NMR and energy transfer techniques, respectively.