NMR ANALYSIS OF RNA STRUCTURE AND DYNAMICS

RNA低聚物的构象和动力学性质将是 检查过了。具体而言，将详细研究的序列包括： (1)三个形成低聚物的双螺旋，其中一个是同源DNA 序列已经被研究过了，一个可能呈现出不同结构的序列 具有不应改变的温度和类似的控制序列，以及 (Ii)R17病毒RNA外壳蛋白结合位点的变异体 对外壳蛋白表现出不同的亲和力。这种蛋白质是 参与R17编码的复制酶基因的翻译抑制。 这些序列将被用作完全碱基配对的RNA和 发夹/凸起环是折叠RNA结构的常见元素， 发生在自然界中。不寻常的结构特征将与 使它们在生物功能中的作用成为可能。 主要工具是核磁共振，该项目寻求 发展和完善质子-质子核的应用。Overhauser 增强作用和碳-13谱。前者将是解决方案，而 后者将产生关于RNA内部动力学的信息。它是 重要的是要把重点放在RNA结构上，它是 在DNA中编码的信息的表达。多年来，这个项目已经 帮助在RNA低聚体和方法的合成方面取得突破 用于指认和评估其复杂的核磁共振谱。这件事也行得通 随着其他实验室的进展使确定三个成为可能- 在这个困难的系统中的空间结构。 该项目将大大促进这一令人振奋的进展 从溶液核磁共振中生成生物分子的详细模型 数据。它还将帮助开发和应用新的方法，在它们可以制造的地方 对基因表达控制的理解上的差异。

The conformational and dynamical properties of RNA oligomers will be examined. Specifically, the sequences that will be studied in detail are: (1) three double helix forming oligomers, one for which the cognate DNA sequence has already been studied, one that may exhibit varying structure with temperature and a similar control sequence that should not vary, and (ii) variants of the binding site for the coat protein of R17 viral RNA that display differential affinities for the coat protein. The protein is involved in the translational repression of the R17-encoded replicase gene. These sequences will be used as models for fully base-paired RNA and hairpin/bulge loops that are common elements of folded RNA structures that occur in nature. unusual structural features will be related as fully as possible to their role in biological function. The principle tool is nuclear magnetic resonance and the project seeks to develop and refine applications of proton-proton nuclear. Overhauser enhancements and carbon-13 spectra. The former will be solution, and the latter will yield information on the internal dynamics of RNA. It is important to focus on RNA structures which are critical biomolecules in the expression of information coded in DNA. Over the years this project has helped create breakthrough in the synthesis of RNA oligomers and methods for assigning and evaluating their complex nmr spectra. This work as well as progress in other labs now make it feasible to determine three- dimensional structures in this difficult system. This project will contribute substantially to the exciting progress being made in the generation of detailed models of biomolecules from solution nmr data. It will also help develop and apply new methods where they can made a difference in the understanding of the control of genetic expression.