BASE STABLE AND COMPOSITE CERAMIC SUPPORTS FOR HPLC

这一合作延续提案的目标是， 基于高度多孔（50%）、巨孔 以及用于这两种用途的二氧化锆的无孔微球 分析和制备规模的蛋白质色谱法，在流化床中 分离器和用于固定化酶和细胞反应器。 总目标 这项工作的主要目的是消除硅基载体的主要问题， 通过用ZrO/2代替SiO/2并利用独特的刘易斯 酸表面化学以及独特的物理性质， 氧化锆，例如其极端的化学、热、物理稳定性，以及 高堆积密度（3.3g/cc）。 多孔、表面改性、高性能 具有>400埃孔的氧化锆颗粒，50个微孔颗粒 对于流化，大孔颗粒（> 1，000埃的孔），磁性的， 和无孔颗粒将被制成并由氮表征 吸附/脱附、孔隙率测定、固态NMR和FTIR方法。 研究了刘易斯碱改性的（氢氧化物，磷酸盐，氟化物， 邻苯二酚，柠檬酸盐），反相（聚丁二烯涂层PBD），葡聚糖- 涂覆（空间排斥、疏水相互作用或离子交换）， 聚乙烯亚胺（PEI）涂覆的（阴离子交换），亲和（亲硫的， 活性染料，伴刀豆球蛋白A-，蛋白A-吸附）支持，所有开始于 以往的拨款将继续发放。 还建议评价 用新聚合物（聚苯乙烯、聚苯乙烯的均聚物、聚苯乙烯的均聚物）涂覆的多孔颗粒 氨基酸、丙烯酸酯、聚烷基二醇）、基于多糖的手性 分离、金属和金属氧化物簇浸渍的氧化锆载体 可用于各种分离如治疗肽， 糖蛋白、核苷和其他重要的生物分子。 无孔 用PEI、PBD或其它聚合物涂覆的氧化锆颗粒将 用于非常快速的蛋白质分离。 优化使用的颗粒 将研究流化床分离器中捕获 免疫球蛋白和重组蛋白 从污染的脂多糖和核酸中纯化。 以前的工作蛋白质吸附氧化锆将扩大调查 用于酶固定化的新型可流化多孔ZrO/2载体和 固定化细菌作为催化剂。

The objectives of this collaborative continuation proposal are to develop a multifaceted family of supports based on highly porous (50%), megaporous and non-porous microspheres of zirconium dioxide to be used for both analytical and preparative scale protein chromatography, in fluidized bed separators and for immobilized enzyme and cell reactors. The overall goal of this work is to eliminate the major problems of silica-based supports by replacing SiO/2 with ZrO/2 and to take advantage of the unique Lewis acid surface chemistry as well as the distinct physical properties of zirconia such as its extreme chemical, thermal, physical stability, and high bulk density (3.3 g/cc). Porous, surface-modified, high performance zirconia particles with >400 angstroms pores, 50 micro- porous particles for fluidization, megaporous particles (>1,000 angstroms pores), magnetic, and non-porous particles will be made and characterized by nitrogen adsorption/desorption, porosimetry, solid state NMR and FTIR methods. Investigation of Lewis base-modified (hydroxide, phosphate, fluoride, catechol, citrate), reversed phase (polybutadiene-coated PBD), dextran- coated (steric exclusion, hydrophobic interaction, or ion exchange), polyethyleneimine (PEI) coated (anion exchange), affinity (thiophilic, reactive dye, concanavalin A-, protein A-adsorbed) supports, all begun on the previous grant will be continued. Also proposed is evaluation of porous particles coated with new polymers (polystyrene, homopolymers of amino acids, acrylates, polyakylglycols), polysaccharide-based chiral separations, metal and metal oxide cluster-impregnated zirconia supports useful for a variety of separations such as therapeutic peptides, glycoproteins, nucleosides and other important biomolecules. Non-porous zirconia particles coated with PEI, PBD, or other polymers will be evaluated for very rapid protein separations. Particles optimized for use in fluidized bed separators will be investigated for capture of immunoglobulin and recombinant proteins of therapeutic interest for rapid purification from contaminating lipopolysaccharide and nucleic acids. Previous work on protein-adsorbed zirconia will be extended to investigate novel fluidizable porous ZrO/2 supports for enzyme immobilization and immobilization of bacteria as catalysts.