FUNCTION OF 95F UNCONVENTIONAL MYOSIN IN DROSOPHILA

在这项建议中概述的研究使用果蝇来确定作用 与肌动蛋白合作来调节肌动蛋白细胞骨架 参与正常的细胞生理。 特别是，这个角色 细胞骨架系统在动态过程中是重要的， 将检查生物体的发育。 本提案的重点 是一种新的非传统肌球蛋白，称为果蝇95F 非常规肌球蛋白，存在于大多数细胞中（如果不是所有细胞） 在苍蝇的整个生命周期中。 有证据表明， 在胚胎早期发育中起重要作用。 审查 这种蛋白质在果蝇生命周期中的功能， 利用该模型中可用的各种工具来执行 有机体 首先，基因突变的影响， 将分析该肌球蛋白重链以确定何时以及在哪种情况下， 这种蛋白质在组织中发挥作用。 此外， 将使用体外功能测定、体内功能测定和体内功能测定来检查蛋白质 技术的可视化过程中，这种蛋白质 参与，以及使用抗体和其他 试剂 肌球蛋白家族的机械化学酶含有多种 一组蛋白质，被认为是介导各种重要的 细胞动力学过程 然而，每个人的特殊作用 家庭的许多不同成员仍然不清楚。 很可能是 本申请中提出的实验将提供关于 这种肌球蛋白在各种组织中的特殊作用， 生物体发育的阶段;因此，这些实验将提供 对细胞如何参与生物体功能的重要见解， 并提供关于特定肌动蛋白基过程的信息 在不同的细胞中维持正常功能。

The studies outlined in this proposal use Drosophila to determine the role of proteins that cooperate with actin to mediate the actin cytoskeleton's participation in normal cell physiology. In particular, the role of this cytoskeletal system in dynamic processes that are important for development of the organism will be examined. The focus of this proposal is an novel unconventional myosin, called the Drosophila 95F unconventional myosin, that is present (in most if not all cells) throughout the life cycle of the fly. Evidence suggests that it plays an important role in early development of the embryo. To examine the function of this protein during the life cycle of Drosophila, the studies to be performed utilize a variety of tools available in this model organism. Primarily, the effects of mutation in the gene that encodes this myosin heavy chain will be analyzed to determine when and in which tissues this protein plays a role. In addition, biochemical properties of the protein will be examined, using in vitro assays for function, in vivo techniques for visualization of processes in which this protein participates, and disruption of function using antibodies and other reagents. The myosin family of mechanochemical enzymes contains a diverse group of proteins that are thought to mediate a variety of important cellular dynamic processes. However, the particular role of each of the many different members of the family remains unclear. It is likely the experiments proposed in this application will provide information on the particular role of this myosin in a variety of tissues and at different stages of organism development; thus, these experiments will provide significant insight into how cells participate in organism function, as well as to provide information on the particular actin-based process required in different cells for their normal function.