FETAL AND PLACENTAL METABOLISM OF THREONINE AND ALANINE

这些研究将检查两个胎儿和胎盘的代谢， 氨基酸：1）必需氨基酸，苏氨酸和2）丙氨酸。 两 是潜在的葡萄糖前体 这些研究将比较他们的比率 胎儿和胎盘的供应 要求. 这些研究将长期进行， 插管妊娠绵羊，在稳态无应激条件下研究 条件 多示踪剂方法将用于研究其 胎盘、胎儿和胎儿肝脏 代谢是可以分离的。 氧化率等离子体处理率 肝脏对这些氨基酸的净摄取和代谢将 在正常条件下和两种不同的模型中进行了研究， 胎儿宫内发育迟缓，一种与血糖降低有关 一种是胎儿分娩和葡萄糖生成，另一种是正常葡萄糖 交付. 净示踪剂和示踪剂通量将被测量进出 胎儿、胎儿肝脏和子宫胎盘。 碳标记 苏氨酸和丙氨酸代谢的潜在产物也将是 测定，特别是对其他氨基酸，葡萄糖和乳酸。

These studies will examine the fetal and placental metabolism of two amino acids: 1) an essential amino acid, threonine and 2) alanine. Both are potential glucose precursors. The studies will compare their rates of supply to the fetus and placenta with the fetal and placental requirements. The studies will be carried out in chronically catheterized pregnant sheep, studied under steady state unstressed conditions. Multiple tracer methodology will be used to study their metabolism in fetuses in which placental, fetal and fetal hepatic metabolism can be separated. The oxidation rate, plasma disposal rates and net hepatic uptake and metabolism of these amino acids will be studied under normal conditions and in two different models associated with intrauterine growth retardation, one associated with reduced glucose delivery to the fetus and glucogenesis and the other with normal glucose delivery. Net tracer and tracee fluxes will be measured into and out of the fetus, the fetal liver and the uteroplacenta. Carbon labelling of potential products of threonine and alanine metabolism will also be determined, particularly for other amino acids, glucose and lactate.