ETHANOL EFFECTS ON CNS TISSUE IN VIVO AND IN VITRO

我们将使用电生理和体内电化学技术来 以乙醇为直接膜的急性神经元反应的特征 影响，与突触输入的调制相互作用，或改变 神经通路的活动。酒精暴露对大鼠脑损伤的影响 哺乳动物的中枢神经系统功能将使用这两种方法进行研究 单个神经元动作电位活动的细胞外记录 以及诱发电位和膜的细胞内记录 属性。我们将在体内研究酒精对啮齿类动物大脑的影响 从完整的脑原位和在眼球的多个脑移植中记录， 并在体外使用脑片和分离的眼内脑移植。 我们还将研究酒精对人脑中使用的神经元的作用。 异种移植物。最后，我们将专注于基因变异的表现 差异酒精相关行为作为研究急性酒精中毒的模型系统 乙醇作用、乙醇依赖和乙醇戒断。新方向 我预计这些科学互动可能会影响我的 实验室包括研究酒精对神经元的影响 对于胎儿酒精综合征，酒精对衰老过程的影响， 乙醇参与偏爱的细胞机制和遗传学 依赖，研究生长因子在糖尿病长期影响中的作用 乙醇，以及对细胞内机制的膜片钳研究，如 第二信使系统，调节乙醇的影响。

We will use electrophysiological and in vivo electrochemical techniques to characterize acute neuronal responses to ethanol as direct membrane effects, modulatory interactions with synaptic inputs, or alterations in the activity of neuronal pathways. The effects of ethanol exposure on mammalian central nervous system function will be pursued using both extracellular recording of action potential activity from single neurons as well as evoked potentials, and intracellular recording of membrane properties. We will study ethanol effects in rodent brain using in vivo recording from intact brain in situ and in multiple brain grafts in oculo, and using brain slices and extricated intraocular brain grafts in vitro. We will also study ethanol actions on human neurons using in oculo brain xenografts. Finally, we will focus on genetic variants manifesting differential alcohol-related behavior as model systems for examining acute ethanol actions, ethanol dependence and ethanol withdrawal. New directions which I anticipate that these scientific interactions may take in my laboratory include studies of the neuronal effects of ethanol associated with fetal ethanol syndrome, the effect of ethanol on aging processes, the cellular mechanisms and genetics of ethanol involved in preference and dependence, studies of the role of growth factors in long-term effects of ethanol, and patch-clamp studies of the intracellular mechanisms, such as second messenger systems, mediating the effects of ethanol.