PHTHALOCYANINE PHOTOSENDITIZER EFFICIENCY ON PTK2 CELLS

这个项目的目的是测量荧光量子产额， 分子的荧光寿命、三重态量子产额和三重态寿命 15种光敏剂，并将这些测量与 这15种化合物的体外光灭活效率比较 PTK2大鼠肾上皮细胞中的光敏剂。这个 15种光敏剂是：酞菁(Pc)，它的双和 四磺酸(PCDS，PCTS)； 氯铝酞菁及其四磺酸盐(CIAIPc，CIAIPcts)； 单甲基脱镁叶绿酸甲酯(MPPB)； 天门冬氨酸氯乙酯(MACE)；锡黄红蛋白(SNET)；血卟啉-IX 和Photofrin(PII)，HPD的一种浓缩形式。光物理 测量将在二甲基甲酰胺(DMF)和 光细胞毒性研究将在磷酸盐缓冲盐水中进行 (PBS)，或在可能的情况下，用于氯化衍生物的PBS-火药剂。 PTK2大鼠袋鼠上皮细胞将引入光敏剂 在24小时内，暴露在适当波长的光下 一台Nd-YAG激光器，并评估了每个络合物的光杀灭效率 用台盼蓝试验检测细胞活力。这项实验寻求 提供了一个预测光杀灭效率的模型 基于其光物理参数的光敏剂。 这项研究是对照片物理的第一次全面研究 多种多样的细菌的行为和光细胞毒性效率 光敏剂，包括“新”类和“旧”类光敏剂- 酞菁及其衍生物、叶绿素和氯化物衍生物- 和Photofrin II。假设最好的光敏剂是 那些在高量子产额下拥有长寿命三重态的那些 氧气浓度保持不变。

The aim of this project is to measure the fluorescence quantum yield, fluorescence lifetime, triplet quantum yield and triplet lifetime for fifteen photosensitizers, and correlate these measurements with the comparative in vitro photoinactivation efficiency of these fifteen photosensitizers in PTK2 rat kangaroo epithelial kidney cells. The fifteen photosensitizers are: phthalocyanine (Pc), its di- and tetrasulfonate (PcDs,PcTs); zinc metallated complexes of Pc, PcDs, PcTs; chloroaluminum phthalocyanine and its tetrasulfonate (CIAIPc, CIAIPcTs); napthalocyanine (NPc), ZnNpc, methyl pheophorbide a (Mppb); mono-L- aspartyl chlorin E6 (MACE); tin etiopurpurin (SnEt); hematoporphyrin-IX and Photofrin (PII), a concentrated form of HpD. Photophysical measurements will take place in Dimethylformamide (DMF) and photocytotoxicity studies will take place in phosphate buffered saline (PBS), where possible, or in PBS- cremophor for the chlorin derivatives. Photosensitizers will be introduced to PTK2 rat kangaroo epithelial cells for twenty-four hours, exposed to light at the appropriate wavelength from a Nd-YAG laser, and the photokill efficiency of each complex assessed utilizing the trypan blue test for cell viability. This experiment seeks to provide a model for predicting the photokill efficiency of photosensitizers based on their photophysical parameters. This study represents the first comprehensive study of the photo physical behavior and the photocytotoxicity efficiency of a wide variety of photosensitizers, including "new" and "old" classes of photosensitizers- phthalocyanine and its derivatives, chlorophyll and chlorin derivatives- and Photofrin II. The hypothesis is that the best photosensitizers are those which possess a long-lived triplet state in high quantum yield when the oxygen concentration remains constant.