DISPLAY OF MRNAS FROM NORMAL TUMORIGENIC CELLS

差异基因表达是一个高度调控的过程，它控制着 正常的细胞生长和分化；其异常是潜在的 疾病的起因状态，如癌症。Gen-Hunter公司 目标有两个：(1)提供生物医学研究社区 拥有一整套基于最新技术的试剂系统 被称为差异显示的突破。(2)利用我们的专业知识 寻找疾病的早期诊断标志物或治疗靶点 癌症等州。来自GenHunter的试剂系统已经制作了 Easy中差异表达基因的鉴定和克隆 联系每一位研究人员。差异显示的原理是 在变性聚丙烯酰胺上系统地分布mRNA3‘末端 使用锚定的寡聚脱氧核糖核酸和脱氧核糖核酸的多种组合进行RNA-PCR后的凝胶 随心所欲的酒瓶。这种建议的选择性显示方法修改了 方法用一组基因代替锚定的寡聚脱氧核糖核酸酶基因 家庭专用分隔器与任意分隔器组合，以 只显示那些感兴趣的基因。因此，它是对差异的补充 通过缩小基因搜索的范围而仅允许 要显示的mRNA的编码区。如果成功，这项工作 将导致更多的商用试剂系统用于一般 并将大大加快猎杀许多重要的 致病基因。

Differential gene expression is a highly regulated process which controls normal cell growth and differentiation; its abnormality is the underlying cause of disease states such as cancer. Gen-Hunter corporation's objective is twofold: (1) To provide the biomedical research community with complete line of reagent systems based on the latest technological breakthrough known as Differential Display. (2) To use our expertise to search for early diagnostic markers or therapeutic targets for disease states such as cancer. The reagent systems from GenHunter have made identifications and clonings of differentially expressed genes within easy reach for every researcher. The principle of Differential Display is to systematically distribute mRNA 3' termini on a denaturing polyacrylamide gel after RNA-PCR using multiple combinations of anchored oligo-dT and arbitrary decamers. This proposed Selective Display approach modifies the method by using, instead of anchored oligo-dT primers, a set of gene family-specific decamers in combinations with arbitrary decamers to display only those genes of interest. Thus it complements Differential Display by narrowing down the scope of a gene search while allowing only the coding regions of mRNAs to be displayed. If successful, this work will lead to more lines of commercial reagent systems for the general research community and will greatly speed up hunting down many important disease-causing genes.